Hetero-biosynthesis of the main bioactive components from medicinal fungus Shiraia bambusiccola
10.16438/j.0513-4870.2020-0698
- VernacularTitle:药用真菌竹黄主要活性成分的异源生物合成
- Author:
Ya-long ZHANG
1
;
Le ZHANG
1
;
Jie GAO
1
;
Jian BAI
1
;
Dao-jiang YAN
1
;
Yuan-yuan ZHANG
2
;
You-cai HU
1
Author Information
1. State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
2. School of Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China
- Publication Type:Research Article
- Keywords:
medicinal fungus;
italic>Shiraia bambusiccola;
hypocrellin;
hetero-biosynthesis;
synthetic biology
- From:
Acta Pharmaceutica Sinica
2020;55(7):1691-1698
- CountryChina
- Language:Chinese
-
Abstract:
italic>Shiraia bambusiccola is an important medicinal fungus in China. Hypocrellins with perylenequinone skeleton are main bioactive components of Shiraia bambusiccola, which are widely used in food, medicine, pesticide and other fields as natural photosensitizers. For example, "hypocrellin ointment" has already been used clinically. As a rare and vulnerable species, wild Shiraia bambusiccola resources are very limited. Due to the complex structure and chanllenge in chemical total synthesis of hypocrellins, it is urgent to find an effective strategy to rationally utilize its medicinal value while protecting the wild resources. In this study, a candidate gene cluster hpc was identified in Shiraia sp. cfcc 84681 based on careful bioinformatic analysis. A heterologous expression system for hpc gene cluster was successfully constructed and a mutant strain with high yield of hypocrellins was obtained, which mainly produced hypocrellin A and isohypocrellin A. The main ingredients in the mutant strain are consistent with that in the wild Shiraia bambusiccola. These results provide a new strategy to solve the shortage of wild Shiraia bambusiccola resources.
