Advances in chromatography-based methods for screening active compounds from natural products

VernacularTitle:基于液相色谱分离的天然产物药物活性成分筛选技术研究进展
Author: Jing-yi JIAN ¹ ; Hui-huang CHEN ² ; Qi-sheng HONG ² ; Lü-huan WANG ¹ ; Yu-mei ZHAO ¹ ; Lei LI ¹ ; Ting-ting ZHANG ¹ ; Hai-bo ZHOU ¹ ; Zheng-jin JIANG ¹
Author Information

1. Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou 510632, China
2. Rehabilitation Department of the First Affiliated Hospital of Jinan University, Guangzhou 510630, China
Publication Type:Research Article
Keywords: natural product; active compounds screening; ultrafiltration; magnetic beads based ligand fishing; cellular membrane chromatography; cellular membrane affinity chromatography; immobilized enzyme reactor; on-line bioassay-guided screening; bioassay-guided fractionation
From: Acta Pharmaceutica Sinica 2020;55(7):1504-1510
CountryChina
Language:Chinese
Abstract: Natural products have been a major source of leading compounds in drug discovery. How to effectively screen active compounds from complex matrix remains an interesting topic. In this review, we comprehensively summarized advanced liquid chromatography based approaches in natural products screening, including pre-column, on-column and post-column screening methods. Their advantages, disadvantages and prospect are also discussed.