Effect of early intra-articular injection of alendronate in reducing subchondral bone loss in rat mandibular condyle after ovariectomy
10.12016/j.issn.2096-1456.2017.02.004
- VernacularTitle:关节腔内早期注射阿仑膦酸钠对卵巢摘除大鼠髁突软骨下骨的影响
- Author:
LI Ting
1
;
CHEN Kan
1
;
LAN Ling-li
1
;
REN Xiao-chun
1
;
YING Bin-bin
2
;
ZHU Song-song
1
Author Information
1. Department of Oral and Maxillofacial Surgery, West Stomotalogical Hospital of China, Sichuan University
2. Department of Stomatology, Ningbo First Hospital
- Publication Type:Journal Article
- Keywords:
Subchondral bone;
Temporomandibular joint;
Osteoporosis;
Ovariectomy;
Drug delivery
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2017;25(2):87-92
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effects of intra-articular injection of alendronate on the mandibular condyle in ovariectomized rats.
Methods :Sixty female rats were randomly divided into five groups: ovariectomy with vehicle treatment alone, early alendronate treatment at ovariectomy, late alendronate treatment at 4 weeks after ovariectomy, shamoperation with vehicle treatment, and the normal control rats. The changes in subchondral bone were evaluated by micro-computed tomography (Micro-CT), tartrate-resistant acid phosphatase (TRAP) staining and real-time quantitative polymerase chain reaction (RT-PCR).
Results : Compared with late alendronate treatment, early alendronate treatment improved microstructural properties of the subchondral bone, with higher bone volume ratio (46.4 + 2.5 vs 37.5 + 2.1; P= 0.038), increased trabecular thickness (47.3 + 1.7 vs 34.6 + 1.4; P = 0.029), elevated trabecular number (8.5 + 0.6 vs 6.2 + 0.3; P = 0.041) and lower trabecular separation (30.2 + 1.6 vs 37.7 + 2.6; P = 0.034). Fewer TRAP-positive cells (4.2 + 0.2 vs 6.8 + 0.4; P = 0.019) and a higher OPG/RANKL ratio (0.38 + 0.01 vs 0.25 + 0.03; P = 0.043) in the subchondral bone were observed in the animals with early treatment group compared to late treatment or ovariectomy/vehicle treatment group.
Conclusion :Our results suggest the therapeutic potential of intra-articular alendronate injection in the treatment of osteoporosis-associated temporomandibular disorders.
- Full text:关节腔内早期注射阿仑膦酸钠对卵巢摘除大鼠髁突软骨下骨的影响.pdf
