Functional analysis of Trichinella spiralis serine protease 1.2 by siRNA mediated RNA interference
- Author:
Yang, F.
1
;
Guo, K.X.
1
;
Yang, D.Q.
1
;
Long, S.R.
1
;
Zhang, X.
1
;
Jiang, P
1
;
Cui, J.
1
;
Wang, Z.Q.
1
;
Liu, R.D.
1
Author Information
- Publication Type:Journal Article
- From:Tropical Biomedicine 2020;37(No.2):458-470
- CountryMalaysia
- Language:English
- Abstract: A T. spiralis serine protease 1.2 (TsSP1.2) was identified in the muscle larvae (ML) and intestinal larvae surface/excretory–secretory (ES) proteins by immunoproteomics. The aim of this study was to determine the TsSP1.2 function in the process of T. spiralis intrusion, growth and reproduction by using RNA interference (RNAi). RNAi was used to silence the expression of TsSP1.2 mRNA and protein in the nematode. On 2 days after the ML were electroporated with 2 µM of TsSP1.2-specific siRNA 534, TsSP1.2 mRNA and protein expression declined in 56.44 and 84.48%, respectively, compared with untreated ML. Although TsSP1.2 silencing did not impair worm viability, larval intrusion of intestinal epithelium cells (IEC) was suppressed by 57.18% (P < 0.01) and the suppression was siRNA-dose dependent (r = 0.976). Infection of mice with siRNA 534 transfected ML produced a 57.16% reduction of enteral adult burden and 71.46% reduction of muscle larva burden (P < 0.05). Moreover, silencing of TsSP1.2 gene in ML resulted in worm development impediment and reduction of female fertility. The results showed that silencing of TsSP1.2 by RNAi inhibited larval intrusion and development, and reduced female fecundity. TsSP1.2 plays a crucial role for worm invasion and development in T. spiralis life cycle, and is a potential vaccine/drug target against Trichinella infection.
- Full text:8.2020my1135.pdf
