Isolation and characterization of Pyricularia oryzae isolated from lowland rice in Sarawak, Malaysian Borneo
10.21161/mjm.190423
- Author:
Nor Ain Hussin
- Collective Name:Freddy Kuok San Yeo; Nur Karimah Mohamad; Nur Najwa Hamsein; Tu Anh Vu Thanh; Hung Hui Chung; Lee San Lai;
- Publication Type:Journal Article
- Keywords:
Pyricularia oryzae;
Sarawak rice;
morphological characteristics;
growth rate;
sporulation rate
- From:Malaysian Journal of Microbiology
2020;16(1):58-67
- CountryMalaysia
- Language:English
-
Abstract:
Aims: Rice blast disease caused by Pyricularia oryzae is one of the major biotic diseases of rice in Sarawak, Malaysian
Borneo. This study aims to isolate and characterize rice blast fungus obtained from infected leaf collected from four
different divisions in Sarawak, viz, Miri, Serian, Sri Aman, and Kuching.
Methodology and results: Twelve succeeded isolates were pre-identified as P. oryzae by morphological characteristics
of spores, followed by verification through (internal transcribed spacer) ITS sequencing. The isolates were evaluated for
morphological characteristics, growth rate and sporulation rate, which were grown on two types of media, (filtered
oatmeal agar) FOMA and (potato dextrose agar) PDA. Morphological characterization showed that the colony surface of
the different isolates varied from smooth and fluffy to rough and flattened mycelia; some were with the present of
concentric rings, and some with aerial mycelia. The growth rate and sporulation rate of each isolate varied based on
types of media used. Most of the isolates grew faster on PDA than on FOMA but produced higher number of spores on
FOMA as compared to PDA.
Conclusion, significance and impact of study: This preliminary study showed that there were variations observed
based on morphological and physiological characterization for the different isolates collected in Sarawak, Malaysian
Borneo. This study is the first step towards understanding variation in the population of P. oryzae from Sarawak.
- Full text:20.2020my0574.pdf
