Antibacterial Property of Synsepalum dulcificum Leaves Aqueous Extract against Oral Pathogens and its Chemical Compounds
10.21315/aos2020.15.1.427
- Author:
Hanim Afzan Ibrahim
1
Author Information
1. a School of Dental Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia
- Collective Name:Nur Karyatee Kassima; Noor Azlin Azraini Che Soh; Zulkhairi Othman; Tuan Nadrah Naim Tuan Ismail
- Publication Type:Journal Article
- Keywords:
Synsepalum dulcificum leaves;
antimicrobial activity;
oral pathogens;
gas chromatography mass spectrometry;
broth microdilution assay
- From:Archives of Orofacial Sciences
2020;15(1):55-64
- CountryMalaysia
- Language:English
-
Abstract:
Synsepalum dulcificum (S. dulcificum) commonly known as “miracle fruit” because its berries have the
capability to modify sour taste to the sweet taste when eaten. Beside the berries, S. dulcificum leaves
were also known to possess biological properties such as antioxidant, antimutagenic and antidiabetic
activities. However, the study of its antimicrobial activity against oral pathogen is still lacking. Thus, this
study aimed to evaluate the antibacterial activity of its leaves against cariogenic bacteria and to analyse
its phytochemical compounds. The samples of S. dulcificum leaves were collected in Kelantan, the east
coast region of Peninsular Malaysia and extracted with distilled water using a Soxhlet technique. The
antibacterial activity of the S. dulcificum leaves aqueous extract against Streptococcus mutans (S. mutans),
Streptococcus sobrinus (S. sobrinus) and Lactobacillus salivarius (L. salivarius) was evaluated using the
broth microdilution assay. The identification of the phytochemical compounds was performed using gas
chromatography-mass spectrometry (GC-MS). The antibacterial study showed the minimum inhibitory
concentration of S. dulcificum leaves aqueous extract against S. mutans and S. sobrinus were 16 mg/mL
and 8 mg/mL, respectively. Interestingly, there was no inhibitory effect of S. dulcificum leaves aqueous
extract against L. salivarius. A total of 42 chemical compounds were identified and major identified
bioactive compounds groups were heterocyclic and phenolic compounds. Our results suggested
S. dulcificum leaves aqueous extract has antimicrobial properties against S. mutans and S. sobrinus, but
no inhibitory activity against oral normal flora, with the presence of bioactive compounds has potential
in oral care products application.
- Full text:1.2020my188.pdf
