Study on the application of Wondfo Rapid Diagnostic Kit in the detection of imported malaria in Hubei Province
10.3969/j.issn.1006-2483.2020.02.012
- VernacularTitle:万孚疟原虫检测试剂盒（RDTs）在湖北省输入性疟疾检测中的应用研究
- Author:
Dongni WU
1
;
Jing XIA
1
;
Kaijie LI
1
;
Huaxun ZHANG
1
;
Lingcong SUN
1
;
Xiaorong DONG
1
;
Sujian PEI
1
;
Mumin CAO
1
Author Information
1. Institute of Schistosomiasis Control, Hubei Provincial Center for Disease Control and Prevention, Wuhan 430079, China
- Publication Type:Journal Article
- Keywords:
Imported malaria cases;
RDTs;
Microscopic examination;
Nested PCR
- From:
Journal of Public Health and Preventive Medicine
2020;31(3):46-49
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effectiveness of the application of Wondfo Rapid Diagnostic Kit (RDTs) in the diagnosis of imported malaria cases in the Malaria Reference Laboratory of Hubei Provence. Methods The complete blood samples of malaria cases and negative card deletion cases reported in Hubei Province from January 2015 to June 2018 were collected and retrospectively analyzed. The results of the provincial malaria reference laboratory were used as the standard, and were compared with those results detected by RDTs, microscopic examination and nested PCR. The differences were statistically analyzed. Results A total of 440 complete samples were collected by the Malaria Reference Laboratory of Hubei Provence, of which 418 samples were confirmed as positive, and 22 samples were confirmed as negative. In terms of the identification ability of P. falciparum, RDTs performed the best, with a coincidence rate of 100.00%, and the coincidence rates nested PCR and microscopic examination were 97.49% and 91.40%, respectively. In terms of the identification specificity for another 3 species of Plasmodium (P. vivax, P. ovarian and P. vivax), nested PCR was the best, the microscopy method was the second best, and RDTs was the lowest. Based on the comprehensive analysis of 12 individual indicators, RDTs had the highest score (32), while the microscopic examination and nested PCR scored 24 and 19, respectively. Conclusion RDTs had certain advantages in the detection of malaria, but they had a low identification specificity for different species. Thus, they can be used as auxiliary tools for microscopic examination and widely used in surveillance work after malaria elimination in Hubei Province.
