Study on the effect of chlorine disinfectants on the destruction of New coronavirus nucleic acid
- VernacularTitle:含氯消毒剂对新型冠状病毒核酸破坏效果研究
- Author:
Maoyi CHEN
1
;
Jie HU
1
;
Chunlin MAO
1
;
Tao SHI
1
;
Ting CHEN
1
;
Yujie ZENG
1
;
Bin LI
1
;
Qiong YI
1
;
Hu LI
1
;
Li WANG
1
Author Information
- Publication Type:Journal Article
- Keywords: Chlorine disinfectants; Inactivation effect; New coronavirus; Fluorescent quantitative PCR
- From: Journal of Public Health and Preventive Medicine 2020;31(3):22-24
- CountryChina
- Language:Chinese
- Abstract: Objective To observe the destructive effects of chlorine disinfectants on the nucleic acid of novel coronavirus, and to assess the feasibility of real-time fluorescent quantitative PCR technology for the evaluation of the disinfection effect of novel coronavirus. Methods A suspension quantitative inactivation test was used to observe the inactivation effects of different concentrations of chlorine containing disinfectants and different action times on the novel coronavirus. RT-PCR method was used to detect novel coronavirus nucleic acid for the disinfection effect evaluation. Results The chlorine disinfectants of 1 000 mg/L and 2 000 mg/L could destroy the nucleic acid of ew coronavirusafter application for over 30s. The chlorine disinfectant of 750 mg/L could destroy the nucleic acid of new coronavirusafter application for over 15min. Chlorine disinfectant of 500 mg/L could not completely destroy the nucleic acid of new coronavirusafter 30 min action. Conclusion The disinfection method stipulated in the national prevention and control plan using 1 000mg / L effective chlorine for the epidemic sites of new coronavirus for 30 minutes could completely destroy the nucleic acid of new coronavirus. The Real-time quantitative PCR can be used to evaluate the disinfection effect of new coronavirus.
