Investigation and analysis of the status of new coronavirus contamination in biosafety laboratories
10.3969/j.issn.1006-2483.2020.03.005
- VernacularTitle:生物安全实验室新型冠状病毒污染现状初步调查
- Author:
Yanwei LI
1
;
Qili ZENG
1
;
Yongzhong JIANG
1
;
Bo YU
1
;
Kun CAI
1
;
Xixiang HUO
1
;
Jun LI
1
;
Miaomiao YUE
1
Author Information
1. Hubei Provincial Center for Disease Control and Prevention, Wuhan 430079, China
- Publication Type:Journal Article
- Keywords:
Biosafety laboratory;
New coronavirus;
Contamination status;
Molecular testing
- From:
Journal of Public Health and Preventive Medicine
2020;31(3):19-21
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand the status of new coronavirus contamination in the biosafety laboratory environment, identify key areas prone to contamination, and provide evidence for disinfection of central objects. Methods surfaces of high-frequency contact environment and protective equipment were sampled with moistened sterile cotton swabs after experiment and before disinfection, the results of the one-step real-time reverse transcription polymerase chain reaction (RT-PCR) of open reading frame 1ab and N fragment were used to evaluate the pollution status. Results Environmental surveys found 4 of 217 samples of environmental objects to be positive for new coronavirus RNA, that positive rate was 1.84%. Among them, BSL-3, BSL-2, and BSL-1 were sampled 23, 184, and 10 respectively. The 3 positive samples were from surfaces of nucleic acid extraction room of BSL-2 and from the handles of pass-through box, laboratory door handles and the outer surface of the alcohol watering pot respectively. The 1 positive sample was from the forearm of the protective clothing in BSL-2 laboratory. Conclusion There was a certain degree of virus pollution in key areas of the new coronavirus laboratory. The BSL-2 laboratory has a higher risk of environmental pollution than the BSL-3 and BSL-1 laboratories.
