Optimization and purification of extraction of polysaccharides from <i>Anoecto-chilus roxburghii</i>

Songbai Zhang; Xun Zhang; Wen XU; Wei XU; Zehao Huang; Yu Lin; Shuyun Chen

Return

Optimization and purification of extraction of polysaccharides from Anoecto-chilus roxburghii

VernacularTitle:金线莲多糖的提取优化与纯化
Author: Songbai ZHANG ¹ ; Xun ZHANG ¹ ; Wen XU ¹ ; Wei XU ¹ ; Zehao HUANG ¹ ; Yu LIN ¹ ; Shuyun CHEN ¹
Author Information

1. School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China.
Keywords: response surface methodology; polysaccharide from Anoectochilus roxburghii; ultrasonic extraction; deproteinization
From: Journal of Pharmaceutical Practice 2020;38(4):354-358
CountryChina
Language:Chinese
Abstract: Objective To optimize the process of ultrasonic extraction of polysaccharide in Anoectochilus roxburghii and to investigate the method of protein removal. Methods The extraction rate of polysaccharide was used as the detection index. On the basis of single factor investigation, Box-Behnken experimental design and response surface method were used to optimize the three factors of material-liquid ratio, ultrasonic time and ultrasonic extraction temperature. The five deproteinization methods including Sevage reagent method, TCA method, salt method (NaOH-CaCl₂ and NaOH-NaCl) and hydrochloric acid method were investigated with the retention rate of polysaccharide and protein removal rate. Results The optimal extraction conditions of polysaccharide from Anoectochilus roxburghii were as follows: liquid-to-solid ratio was 10∶1, extraction temperature was 48 ℃ and extraction time was 36 min with extraction 2 times, ultrasonic power was 300 W, the extraction rate was 13.13%. NaOH-CaCl₂ deproteinized methods∶ the loss rate of polysaccharide was 18.74%, and the removal rate of protein was 95.62%. Conclusion Ultrasonic extraction is easy to operate, and the optimized extraction method can achieve a high extraction rate. NaOH-CaCl₂ deproteinization methods can get high protein removal rate and polysaccharide retention rate. This method is suitable for the research and development of the active components of the polysaccharides from Anoectochilus roxburghii.