Determination of propylthiouracil in human plasma by UPLC-MS/MS
10.12206/j.issn.1006-0111.202001063
- VernacularTitle:UPLC-MS/MS法测定人血浆中丙硫氧嘧啶的含量
- Author:
Qunying LI
1
;
Lin LING
2
;
Chengjian LI
1
;
Jin ZHOU
1
;
TianTian LI
3
;
Liang ZHAO
1
;
Lei LÜ
4
Author Information
1. Department of Pharmacy, Shanghai Baoshan Luodian Hospital, Shanghai 201908, China.
2. College of Traditional Chinese Medicine，Zhejiang University of Technology, Hangzhou 310014, China.
3. Baoshan Dachang Community Health Center. Shanghai 200442, China.
4. Department of Pharmacy, Shanghai Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China.
- Keywords:
UPLC-MS/MS;
propylthiouracil;
plasma;
content determination
- From:
Journal of Pharmaceutical Practice
2020;38(4):350-353
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the determination of propylthiouracil in human plasma by UPLC-MS/MS and provide methodological basis for therapeutic drug monitoring (TDM) and bioequivalence test (BE) in clinical. Methods The chromatographic separation was performed on an Agilent SB-C18 column (4.6 mm×150 mm, 5 μm), the mobile phase was methanol and water containing 0.1% formic acid (80∶20, V/V), isocratic elution. MS condition was optimized in the positive ion detection mode by multiple reaction monitoring (MRM), along with the Agilent JetStream electrospray source interface (AJS-ESI). The precursors to the product ion transitions were m/z 171.1→112.1 for propylthiouracil and m/z 176.1→117.0 for the internal standard (IS). Results The calibration curve was linear in the range of 10−5 000 ng/ml for propylthiouracil in human plasma, r=0.999 3. The intra-day and inter-day precision and accuracy were good (RSD<10%，RE<±10%). The matrix effect of different concentrations was less than 110% and the coefficient of variation was less than 5%. The average recovery of different concentrations was 101.60%−113.56%, which conformed with the requirement of methodological validation. Conclusion The method is rapid, sensitive and accurate, which can be used for the determination of propylthiouracil in human plasma.
