Identification of Chemical Components in Actinidia chinensis Root by UHPLC-Q-TOF-MS/MS
- VernacularTitle:基于UHPLC-Q-TOF-MS/MS技术鉴定藤梨根的化学成分
- Author:
Fangchao CHEN
1
,
2
;
Shouhong GAO
3
;
Zhipeng WANG
3
;
Hong YANG
3
;
Qingsheng SHUN
2
;
Xinhua SONG
4
;
Yixin XU
2
Author Information
1. Graduate School，Shanghai University of TCM，Shanghai 201213，China
2. School of Pharmacy，Shanghai University of Medicine & Health Sciences，Shanghai 201318，China
3. Dept. of Pharmacy，Changzheng Hospital Affiliated to Naval Medical University，Shanghai 200003，China
4. College of Chemistry and Bio-engineering，Yichun Univ ersity，Jiangxi Yichun 336000，China
- Publication Type:Journal Article
- Keywords:
Actinidia chinensis root;
UHPLC-Q-TOF-MS/MS;
Chemical component;
Identification
- From:
China Pharmacy
2020;31(14):1725-1731
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To identi fy chemical components of Actinidia chinensis root rapidly ，and to provide reference for further material basis and quality control study of the crude medicine. METHODS ：UHPLC-Q-TOF-MS/MS technique was used to detect chemical components of A. chinensis root. The separation was performed on Waters XSelect HSS T 3 column with mobile phase consisted of 0.1％ formic acid acetonitrile solution- 0.1％ formic acid water solution （gradient elution ）at the flow rate of 0.3 mL/min. The column temperature was set at 40 ℃，and sample size was 3 μL. Electrospray ion source was adopted，the data was collected under negative ion mode ；the scanning range was m/z 50-1 500；the drying gas temperature was 350 ℃，the atomizing air pressure was 45 psi，the capillary voltage was 3 500 V，and sheath gas temperature was 350 ℃. According to the information of excimer ion and secondary fragment ion ，the chemical components were identified by combining with the relevant literature ，the retention time of the reference substance and the law of mass spectrometry cracking. RESULTS & CONCLUSIONS ：Totally 58 chemical components was identified ，which included 16 pentacyclic triterpenes （such as hydroxyasiatic acid ，asiatic acid ，maslinic acid，corosolic acid ，oleanic acid ，ursolic acid ，etc.），12 flavonoids（such as rutin ，quercitrin，cynaroside，astragalin，etc.），17 organic acids （such as cryptochlorogenic acid ，chlorogenic acid ，isochlorogenic acid A ，isochlorogenicacid C ，etc.）. There were 9 components（such as procydanidin B 1，B2 and luteolin ，etc.）identified for the first time in A. chinensis root. UHPLC-Q-TOF-MS/ MS technique can be used for the rapid identification of chemical components in A. chinensis root.
