Study on Metabolism of Miao Medicine Laportea bulbifera Extract in Isolated Human Intestinal Flora
- VernacularTitle:苗药红禾麻提取物在离体人肠道菌群中的代谢研究
- Author:
Cun XUE
1
;
Dan WU
2
;
Zipeng GONG
1
;
Siying CHEN
1
;
Juan TANG
3
;
Yueting LI
1
;
Aimin WANG
1
;
Yongjun LI
1
;
Yanyu LAN
1
;
Yonglin WANG
1
Author Information
1. Guizhou Provincial Key Laboratory of Pharmaceutical preparations /School of Pharmacy，Guizhou Medical University，Guiyang 550004，China
2. Key Laboratory of Natural Products Chemistry of Guizhou Provincial Science City，Guiyang 550014，China
3. Phase Ⅰ Ward，the Affiliated Hospital of Guizhou Medical University，Guiyang 550004，China
- Publication Type:Journal Article
- Keywords:
Laportea bulbifera;
Isolated human intestinal flora;
Metabolism;
UPLC-Q-TOF/MS;
Identification
- From:
China Pharmacy
2020;31(14):1683-1690
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To explore the metabolic charact eristics of Miao medicine Laportea bulbifera extract in isolated human intestinal flora. METHODS ：L. bulbifera was extracted with 70％ ethanol reflux extraction. After concentration，extraction with n-butanol and drying ，L. bulbifera extract was obtained. Taking 0.05 g/mL L. bulbifera extract 1 mL mixed with isolated human intestinal flora fluid 10 mL and cultured for 36 h in anaerobic environment （setting up blank control without drugs or human intestinal bacterial solution ），so as to simulate the metabolic process of the extract in human intestine. The metabolites were detected by UPLC-Q-TOF/MS. The determination was performed on Agilent Eclipse Plus C 18 RRHD column with mobile phase consisted of 0.01％ formic acid water solution- 0.01％ formic acid acetonitrile solution （gradient eluetion ）at the flow rate of 0.25 mL/min. The column temperature was set at 40 ℃，and the sample size was 1 µL. ESI detection was adopted and scanned by negative ion mode （ESI－）；the capillary voltage was 4.5 kV，the ion source temperature was 120 ℃，the collision energy was 15-32 V，and the scanning range was m/z 50-1 000. The “Strip”module of MassLynx V 4.1 software was used to analyze the differential chromatograms between the reaction solution and the blank control of L. bulbifera extract. Mass spectrum data and UNIFI so ftware were used to predict relative molecular weight and formula ；based on the information of substance control and related literature reports ， the structure and biotransformation pathway of L. bulbifera metabolites in isolated human intestinal flora were predicted and analyzed. RESULTS & CONCLUSIONS ： A total of 3 prototype ： products（rutin，quercetin，kaempferol-3-O-rutinoside）and 22metabolites （mainly the metabolites of quercetin ，mono- caffeoylquinic acid ，isoquercitrin，etc.） were detected after metabolized in isolated human intestinal flora. Itsbiotransformation pathway is phase Ⅰ reaction，which mainly consisted of reduction ，oxidation and hydrolysis.
