Simultaneous determination of illicit drugs and their metabolites in wastewater by SPE-UPLC-MS/MS
10.11665/j.issn.1000-5048.20200308
- VernacularTitle:SPE-UPLC-MS/MS同时检测污水中多种滥用药物及其代谢物
- Author:
Jingyuan WANG
1
;
Chenzhi HOU
;
Zhendong HUA
;
Tingting ZHANG
;
Mengxiang SU
;
Hui XU
;
Youmei WANG
;
Bin DI
Author Information
1. 中国药科大学药物分析系
- Publication Type:Journal Article
- Keywords:
illicit drugs;
wastewater;
solid phase extraction (SPE);
ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS);
content determination
- From:
Journal of China Pharmaceutical University
2020;51(3):305-312
- CountryChina
- Language:Chinese
-
Abstract:
A quantitative analysis method based on solid phase extraction-ultra performance liquid chromatography-tandem mass spectrometry (SPE-UPLC-MS/MS) for simultaneous determination of illicit drugs and their metabolites in wastewater was established. Samples filtered at pH of 2 and spiked with internal standard were loaded to Oasis Prime MCX cartridges for solid-phase extraction. The samples were washed with 4 mL of methanol and eluted with 4 mL of 5% ammonia in acetonitrile before reconstituting with 0.1% formic acid/water solution. ZORBAX Eclipse Plus C18 column was used for chromatography, and gradient elution was performed with 0.1% formic acid/water solution and acetonitrile as mobile phase. The samples were then detected by electrospray ionization (ESI) in positive ion mode, and multiple reaction monitoring mode (MRM) was adopted for quantitative analysis. All analytes had a good linear relationship (r ≥ 0.993 2) within the range of their respective standard curve; the limit of quantification was 1 ng/L (except amphetamine at 2.5 ng/L); the extraction recovery ranged from 82.13% to 99.96%; and the intra- and inter-day precisions were less than 9.43%. The method is accurate, reliable and reproducible, and is suitable for the quantitative determination of illicit drugs and their metabolites in wastewater and can provide an analytical method for real-time monitoring of drug abuse.
