Effect of lncRNA MEG3 on the malignant biological behaviors of cervical cancer standard regimen for advanced colorectal cancer
10.3872/j.issn.1007-385x.2020.07.002
- VernacularTitle:lncRNA MEG3通过miR-9-5p/SOCS5轴对宫颈癌细胞恶性生物学行为
- Author:
ZHANG Yan
1
;
LI Feng
2
;
XU Wan
3
;
ZHANG Li
4
;
DUAN Yating
4
;
YU Zuoqiong
4
Author Information
1. 1a. Department of Gynaecology
2. 1b. Department ofOncology, Chongqing Hospital of Traditional Chinese Medicine, Chongqing 400011, China
3. 2. 7th Medical Center, General Hospital of PLA, Beijing 100039, China
4. 1a. Department of Gynaecology,
- Publication Type:Journal Article
- Keywords:
lncRNA MEG3;
miR-9-5p/SOCS5 axis;
cervical cancer;
HeLa cell;
SiHa cell;
proliferation;
migration;
invasion;
epithelialmesenchymal transition (EMT)
- From:
Chinese Journal of Cancer Biotherapy
2020;27(7):725-734
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective: To explore the regulatory effect of lncRNA maternal imprinting gene 3 (MEG3) on proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) of cervical cancer cells via miR-9-5p/SOCS5 axis. Methods: A total of 20 pairs of cancer and para-cancerous tissue specimens resected from cervical cancer patients in Chongqing Hospital of Traditional Chinese Medicine from January 2017 to June 2019 were collected for this study. Using liposome transfection technology, pcDNA3.1-MEG3,si-MEG3, miR-9-5p mimics, miR-9-5p inhibitor and their control plasmids were transfected into cervical cancer HeLa and SiHa cells respectively to construct overexpression and silence cell model. qPCR was used to detect the expression levels of MEG3, miR-9-5p and SOCS5 in cervical cancer tissues and cell lines. CCK-8 method and Transwell chamber method were used to detect cell proliferation, migration and invasion ability. The expression levels of E-cadherin and vimentin in cells were detected by cellular immunofluorescence experiments. Target genes were predicted through the Online Bioinformatics TargetScan database. Dual luciferase reporter gene assay was used to verify the targeting relationship between miR-9-5p and MEG3, SOCS5, respectively. Results: Compared with para-cancerous tissues and cervical epithelial HcerEpic cells, the expressions of MEG3 and SOCS5 were significantly down-regulated and the expression of miR-9-5p was significantly up-regulated in cervical cancer tissues and cell lines (all P<0.01). TargetScan database analysis and Dual luciferase reporter gene assay confirmed the targeting relationship between miR-9-5p and MEG3 or SOCS5. MEG3 and SOCS5 significantly inhibited while miR-9-5p significantly promoted cell proliferation, migration and invasion ability (all P<0.01). MEG3 and SOCS5 promoted E-cadherin expression and inhibited vimentin expression, while miR-9-5p inhibited
E-cadherin expression and promoted vimentin expression (P<0.05 or P<0.01). Conclusion: lncRNA MEG3 regulates proliferation,
migration, invasion and EMT of cervical cancer cells via miR-9-5p/SOCS5 axis.
- Full text:20200702.pdf
