Effects of Dendrobium officinale flowers on testivular tissue and sperm quality in parent and offspring rats
10.19485/j.cnki.issn2096-5087.2020.05.003
- VernacularTitle:铁皮石斛花对亲代及子代大鼠睾丸组织和精子质量的影响
- Author:
FU Jianyun
1
;
SONG Yanhua
;
ZHENG Yunyan
;
ZHU Zhoujing
;
HE Jiawei
;
XIA Yong
Author Information
1. Zhejiang Provincial Center for Disease Control and Prevention
- Publication Type:Journal Article
- Keywords:
Dendrobium officinale flowers rat sperm testis safety evaluation
- From:
Journal of Preventive Medicine
2020;32(5):442-445
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To learn the effects of Dendrobium officinale flowers on testivular tissue morphology and sperm quality in parent and offspring rats,so as to provide reference for safety evaluation of Dendrobium officinale flowers.
Methods:The 40 SD rats was randomly divided into the low-,middle-,high-dose and the control group,given 2.0,4.0,6.4 and 0 g/kgbw Dendrobium officinale flowers,respectively. After three months,the body weight,mass and organ/body coefficients of testis and epididymis of parent （P） and offspring （F1,F2） rats were measured;the number,activity and deformity of sperms were observed under microscope;the changes of testis and epididymis were observed by hematoxylin eosin staining.
Results:There were no significantly statistical differences in the body weight,mass and organ/body coefficients of testis and epididymis,sperm quantity,sperm motility rate among four groups of P、F1、F2 male rats （P>0.05）. There were no significantly statistical differences in sperm malformation rate between the high-dose group and the control group （P>0.05）. There was no significant change in testis and epididymis of P,F1 and F2 male rats.
Conclusion:Dendrobium officinale flowers did not show obviously adverse effects on testivular tissue morphology and sperm quality in parent and offsping rats.
