ASSOCIATION OF TUMOR ANGIOGENIC CELLS (CD133+/VEGFA+) AND CIRCULATING CANCER STEM CELLS (CD133+/VEGFR2-) IN ASTROCYTIC GLIOMA PATIENTS

Das P; Naing NN; Wan-arfah N; Noorjan K; Kueh YC; Rasalingam K

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ASSOCIATION OF TUMOR ANGIOGENIC CELLS (CD133+/VEGFA+) AND CIRCULATING CANCER STEM CELLS (CD133+/VEGFR2-) IN ASTROCYTIC GLIOMA PATIENTS

Author: Das P ¹ ; Naing NN ² ; Wan-Arfah N ² ; Noorjan K ³ ; Kueh YC ^{4
,

5} ; Rasalingam K ⁶
Author Information

1. Faculty of Medicine, SEGi University, No. 9, Jalan Teknologi, Taman Sains Selangor, Kota Damansara, PJU 5, 47810 Petaling Jaya, Selangor, Malaysia
2. Institute for Community (Health) Development (i-CODE), Universiti Sultan Zainal Abidin, Block E, Level 1, Gong Badak Campus, 21300 Kuala Nerus, Terengganu, Malaysia
3. Department of Psychiatry, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
4. Unit of Biostatistics &
5. Research Methodology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia, Department of Psychiatry, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia
6. Department of Neuroscience, Hospital Kuala Lumpur, 50586 Jalan Pahang, Kuala Lumpur, Malaysia
Publication Type:Journal Article
Keywords: Glioma; Astrocytic; Astrocytomas; Angiogenic Cells; Brain Tumors
From:Journal of University of Malaya Medical Centre 2019;22(2):31-38
CountryMalaysia
Language:English
Abstract: Background: Astrocytic gliomas are the most common primary brain tumors that developed from glial origin. The angiogenic cell population from brain tumor enhances the recruitment of circulating cancer stem cells homing towards tumor site. Objectives: This study aimed to investigate the tumor angiogenic cell population that stained with CD133+ and VEGFA+ markers and its association with circulating cancer stem cell (CD133+/VEGFR2-) population in the peripheral blood mononuclear cells (PBMCs) of astrocytic glioma patients.Methods: A total of 22 astrocytic glioma patients from Hospital Universiti Sains Malaysia who consented to the study were included. Tumors (n=22) were sliced and stained with CD133+ and VEGFA+ angiogenic markers and counter stained with DAPI. The circulating cancer stem cells (CD133+/VEGFR2-) in PBMCs (n=22) were quantified using FACS based on the expression of CD133 and VEGFR2 markers. The paired t-test and Pearson correlation were used for the data analysis.Results: The percentage of angiogenic cell population was significantly higher in brain tumor compared to adjacent normal brain tissue (1.25 ± 0.96% vs. 0.74 ± 0.68%; paired t-test=2.855; df=21, p = 0.009). Positive correlation was found between the angiogenic cells of brain tumor tissue and adjacent normal brain tissue (Pearson correlation, r = 0.53, p = 0.011). Significant positive correlation was found between angiogenic cells in glioma tumor and cancer stem cells in peripheral circulating systems of astrocytic glioma patients (Pearson correlation, r = 0.42, p = 0.049).Conclusion: Angiogenic cells in the brain tumor resident promote the recruitment of circulating cancer stem cells homing to the tumor site and induce the proliferation and growth of the tumor in astrocytic glioma patients.
Full text:10.2019.my005.pdf