Studies on Alkaline Phosphatase Isoenzyme in the Serum and Organs of the Rat.
10.3349/ymj.1984.25.2.142
- Author:
Won Joon KIM
1
;
Hye Seong CHO
;
Sa Suk HONG
Author Information
1. Department of Pharmacology, Yonsei University College of Medicine, Seoul, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Alkaline phosphatase isoenzyme;
Isoelectro focusing;
Isoelectric points
- MeSH:
Alkaline Phosphatase/analysis*;
Alkaline Phosphatase/blood;
Animal;
Isoenzymes/analysis*;
Isoenzymes/blood;
Rats
- From:Yonsei Medical Journal
1984;25(2):142-148
- CountryRepublic of Korea
- Language:English
-
Abstract:
Isoenzymes of alkaline phosphatase from purified extracts of liver, intestine, pancreas and bone of rats were determined by their isoelectric points and compared with those from serum. 1) The extracts obtained from homogenized tissues were centrifuged at 65,000xg and filtered through an Ultrogel AcA 34 column. Among the three major peaks obtained by gel filtration, the second peak fractions were further separated by isoelectric focusing. Isoenzymes of alkaline phosphatase were found only in the second peak. 2) Isoenzymes of alkaline phosphatase were distinguishable with pH 3.5-10 ampholytes. When pH 3-6 ampholytes were used, isoenzymes were more clearly separated, e.g., 4in serum, 5 in intestine and 2 each in the liver, pancreas, and bone. 3) Comparing the bands of the isoenzymes of alkaline phosphatase to those of serum, only the band with 5.04 pI was the same between serum and intestine. These results indicate that several forms of alkaline phosphatase, even though all are from the rat, may exist; and some of the isoenzymes of alkaline phosphatase found in the serum originated from the intestine.
