Comparative Genetic Characterization of Plasmids of Agrobacterium Species Isolated in Korea.
10.12701/yujm.1984.1.1.41
- Author:
Jung Hye KIM
;
Yong Bum KOO
;
Ki Young LEE
;
Jae Kyu CHUNG
- Publication Type:Original Article
- MeSH:
Agrobacterium tumefaciens;
Agrobacterium*;
Carcinogenesis;
Centrifugation, Density Gradient;
Cesium;
DNA;
DNA, Bacterial;
Flowers;
Korea*;
Plant Tumor-Inducing Plasmids;
Plant Tumors;
Plants;
Plasmids*;
Populus;
Rosa;
Soil;
Solar System;
Wounds and Injuries
- From:Yeungnam University Journal of Medicine
1984;1(1):41-48
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The soil bacterium Agrobacterium tumefaciens is a plant pathogen that causes crown gall tumors by infecting the wounded dicotyledonous plants and subsequent integration of bacterial DNA into plant nuclear DNA. Virulent A. tumefaciens strains harbor a large Ti (tumor–inducing) plasmid that carries genes essential for tumorigenesis. In the present study, 13 strains (Malus pumila Mill; A₁₋₃, Populus monilifera; W₁₋₆, Populus tomentiglandlosa; P₁₋₃ and Rosa species; R₁) of Agrobacterium isolated in korean crown gall tumors and plasmids were observed in 6 strains (W₂, W₃, W₆, P₁, P₃ and A₂). The test for crown gall tumor formation was resulted only in ATCC15955 and KW2 strains inoculated into the stem of sun flower and the development was observed for 4 and 6 weeks after inoculation. Above two Ti plasmids (pTi) were purified by cesium chloride-ethidium bromide density gradient centrifugation and digested with restriction enzyme and fragments of pTiATCC15955 and pTiKW₂ observed by EcoR I ; 25&27, Hind III ; 23&21, BamH I ; each 20 and Hpa I ; 12&27. And sizes of pTiATCC15955 and and pTiKW₂ calculated as 200 and 87 kbases. Octopine was isolated from tumor tissue (W₁₋₆ and P₁₋₃) and these strains confirmed as octopine type.
