Comparative Study on the Effect of Raw Product and Different Processed Products of Gekko gecko on Kidney- yang Deficiency Model Mice Induced by Adenine
- VernacularTitle:蛤蚧生品及不同炮制品对腺嘌呤致肾阳虚模型小鼠的改善作用比较
- Author:
Xinhui HUANG
1
;
Xiaoshan WANG
2
;
Shuling LIU
2
;
Yanchun WU
2
;
Bei ZHOU
2
;
Bing ZHANG
2
;
Guiyu XIONG
2
Author Information
1. Dept. of Pharmacy，Guangxi International Zhuang Medical Hospital，Nanning 530021，China
2. College of Pharmacy，Guangxi University of TCM，Nanning 530200，China
- Publication Type:Journal Article
- Keywords:
Gekko gecko;
Raw product;
Processed product;
Kidney-yang deficiency;
Adenine;
Mice
- From:
China Pharmacy
2020;31(13):1608-1612
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To compare the effects of raw pro duct and different processed products of Gekko gecko on kidney-yang deficiency model mice induced by adenine. METHODS ：Totally 100 mice were randomly divided into blank group （n＝10）and modeling group （n＝90）. Modeling group was given adenine （50 mg/kg）intragastrically for 10 days to induce kidney-yang deficiency model ；blank group was given normal saline （0.2 mL/10 g）intragastrically. After modeling ，70 mice were randomly divided into model group ，positive group （Jinkui shenqi pill ，6.4 g/kg），G. gecko crude product group （1.2 g/kg）， wine-processed G. gecko group（1.2 g/kg）and oil-processed G. gecko group（1.2 g/kg）according to body weight and symptoms of kidney-yang deficiency ，with 14 mice in each group. Blank group and model group were given constant volume of normal saline intragastrically；administration groups were given relevant medicine intragastrically （0.2 mL/10 g），once a day ，for consecutive 14 d. During the experiment ，the symptoms and signs of mice in each group were observed. After last medication ，renal index ，testis index and serum levels of T ，CORT，BUN and Cr were measured ；HE staining method was used to observe the pathological changes of renal tissue of mice in each group. RESULTS ：Compared with blank group ，the mice in the model group suffered from performance of kidney-yang deficiency ，such as weight loss ，crouch and arch back ，chills and cold limbs ，and sparse body hair ， while renal index and serum levels of BUN and Cr were increased significantly （P＜0.01）. In renal tissue ，there were BA28117 pathological damages such as irregular arrangement of renal KY2016YB211tubular epithelial cells ，light staining of nucleus and edema of cytoplasm. Compared with model group ， performance of kidney-yang deficiency was improved to different extents in G. gecko crude product group and processed product groups（especially in wine-processed G. gecko group）；serum levelsof BUN and Cr were decreased significantly （P＜0.05 or P＜0.01）；pathological damage of renal tissue was alleviated in different degrees. In addition ，body weight of mice was increased significantly in G. gecko processed products groups （P＜0.01），and renal indexes of mice were decreased significantly in G. gecko crude product group and processed products groups （P＜0.05 or P＜0.01）. Compared with G. gecko crude product group ，renal index ，serum levels of BUN and Cr were significantly decreased in wine-processed G. gecko group（P＜0.01），and serum level of Cr was significantly decreased in oil-processed G. gecko group（P＜ 0.05）. CONCLUSIONS ：G. gecko crude product ，wine-processed G. gecko and oil-processed G. gecko all show a certain improvement effect on kidney-yang deficiency mice induced by adenine ，especially wine-processed G. gecko .
