Comparative Study on the Effect of Raw Product and Different Processed Products of Gekko gecko on Kidney- yang Deficiency Model Mice Induced by Adenine
- VernacularTitle:蛤蚧生品及不同炮制品对腺嘌呤致肾阳虚模型小鼠的改善作用比较
- Author:
Xinhui HUANG
1
;
Xiaoshan WANG
2
;
Shuling LIU
2
;
Yanchun WU
2
;
Bei ZHOU
2
;
Bing ZHANG
2
;
Guiyu XIONG
2
Author Information
1. Dept. of Pharmacy,Guangxi International Zhuang Medical Hospital,Nanning 530021,China
2. College of Pharmacy,Guangxi University of TCM,Nanning 530200,China
- Publication Type:Journal Article
- Keywords:
Gekko gecko;
Raw product;
Processed product;
Kidney-yang deficiency;
Adenine;
Mice
- From:
China Pharmacy
2020;31(13):1608-1612
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To compare the effects of raw pro duct and different processed products of Gekko gecko on kidney-yang deficiency model mice induced by adenine. METHODS :Totally 100 mice were randomly divided into blank group (n=10)and modeling group (n=90). Modeling group was given adenine (50 mg/kg)intragastrically for 10 days to induce kidney-yang deficiency model ;blank group was given normal saline (0.2 mL/10 g)intragastrically. After modeling ,70 mice were randomly divided into model group ,positive group (Jinkui shenqi pill ,6.4 g/kg),G. gecko crude product group (1.2 g/kg), wine-processed G. gecko group(1.2 g/kg)and oil-processed G. gecko group(1.2 g/kg)according to body weight and symptoms of kidney-yang deficiency ,with 14 mice in each group. Blank group and model group were given constant volume of normal saline intragastrically;administration groups were given relevant medicine intragastrically (0.2 mL/10 g),once a day ,for consecutive 14 d. During the experiment ,the symptoms and signs of mice in each group were observed. After last medication ,renal index ,testis index and serum levels of T ,CORT,BUN and Cr were measured ;HE staining method was used to observe the pathological changes of renal tissue of mice in each group. RESULTS :Compared with blank group ,the mice in the model group suffered from performance of kidney-yang deficiency ,such as weight loss ,crouch and arch back ,chills and cold limbs ,and sparse body hair , while renal index and serum levels of BUN and Cr were increased significantly (P<0.01). In renal tissue ,there were BA28117 pathological damages such as irregular arrangement of renal KY2016YB211tubular epithelial cells ,light staining of nucleus and edema of cytoplasm. Compared with model group , performance of kidney-yang deficiency was improved to different extents in G. gecko crude product group and processed product groups(especially in wine-processed G. gecko group);serum levelsof BUN and Cr were decreased significantly (P<0.05 or P<0.01);pathological damage of renal tissue was alleviated in different degrees. In addition ,body weight of mice was increased significantly in G. gecko processed products groups (P<0.01),and renal indexes of mice were decreased significantly in G. gecko crude product group and processed products groups (P<0.05 or P<0.01). Compared with G. gecko crude product group ,renal index ,serum levels of BUN and Cr were significantly decreased in wine-processed G. gecko group(P<0.01),and serum level of Cr was significantly decreased in oil-processed G. gecko group(P< 0.05). CONCLUSIONS :G. gecko crude product ,wine-processed G. gecko and oil-processed G. gecko all show a certain improvement effect on kidney-yang deficiency mice induced by adenine ,especially wine-processed G. gecko .