Comparison of Chemical Constituents Differences between Angelicae dahuricae with and without Sulphur- fumigation and Selection of Chemical Markers Based on 1H-NMR Metabolomics
- VernacularTitle:基于1H-NMR代谢组学技术比较熏硫和未熏硫白芷的化学成分差异及化学标志物筛选
- Author:
Yun DONG
1
;
Meigui TIAN
1
;
Rongwei TANG
1
;
Ling TANG
1
;
Chengcheng ZHAO
2
;
Yi ZHANG
2
;
Gang FAN
2
Author Information
1. Dept. of Pharmacy，Dazhou Vocational College of TCM，Sichuan Dazhou 635000，China
2. College of Ethnic Medicine，Chengdu Uni versity of TCM，Chengdu 611137，China
- Publication Type:Journal Article
- Keywords:
Angelicae dahuricae;
Sulphur-fumigation;
1H-NMR metabolomics;
Chemical markers;
Identification
- From:
China Pharmacy
2020;31(13):1557-1561
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To study the diff erences in the overall chemical constituents of Angelicae dahuricae with and without sulphur-fumigation，and to select chemical markers that can be used for identification. METHODS ：Each 16 batches of A. dahuricae with and without sulphur-fumigation were selected as samples. Deuterated methanol-deuterium oxide （1 ∶ 0.2，V/V）was used as extraction solvent ，and 1H-NMR metabolomics was used to detect the primary and secondary metabolites in A. dahuricae . In addition ，visual analysis ，principal component analysis （PCA）and t-test were used to compare the component differences of A. dahuricae by two kinds of pretreatment methods. RESULTS ：A total of 19 chemical constituents such as coumarins ，amino acids and sugars were simultaneously detected and identified from two kinds of A. dahuricae . Visual analysis showed that the chemical profiles of the two kinds of A. dahuricae were similar ，but their coumarins and carbohydrates were quite different in 1H-NMR signal intensity. PCA analysis showed that all samples could be divided into two categories according to different treatment methods. The results of t-test showed that the contents of oxypeucedanin ，imperatorin，glucose and sucrose of A. dahuricae were decreased significantly after sulphur fumigation （P＜0.01 or P＜0.001），while the contents of alanine and leucine were significantly increased （P＜0.01 or P＜0.001）. There was no statistical significance in the contents of other 13 kinds of components （P＞0.05）. CONCLUSIONS：There are obvious differences in the 6 chemical compounds of oxypeucedanin ，imperatorin，glucose，sucrose， alanine and leucine in A. dahuricae with and without sulphur-fumigation ，which can be used as chemical markers for the identification of sulphur-fumigated A. dahuricae in the market.
