The effect of PKD1 gene on autophagy in aortic smooth muscle cells
- VernacularTitle:PKD1 基因对主动脉平滑肌细胞自噬的影响
- Author:
Guifang YANG
1
;
Xiangping CHAI
1
;
Wen PENG
1
;
Yang ZHOU
1
;
Lijuan SHENG
2
Author Information
1. Department of Emergency Medicine, The Second Xiangya Hospital, Central South University, Changsha, 410011, P.R.China
2. Department of Neurology, The Second Xiangya Hospital, Central South University, Changsha, 410011, P.R.China
- Publication Type:Journal Article
- Keywords:
PKD1 gene;
vascular smooth muscle cell;
lentivirus;
autophagy
- From:
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
2020;27(05):569-573
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of PKD1 gene on mouse aortic smooth muscle (MOVAS) cells autophagy. Methods The shRNA and over-expression lentiviral vectors for the target gene of PKD1 were constructed. MOVAS cells were infected by a number of successful packaging shRNA (PKD1 knockdown) or ETS-1 (PKD1 over-expressing) lentiviral vectors, and qPCR was used to test interference and over-expressing effects. Then qPCR and Western blotting were used to detect the expression levels of autophagy markers including Atg5, Beclin1 and LC3 in control group, shPKD1 group and ETS-1 group. Results Compared with the control group, PKD1 mRNA level was decreased in the shPKD1 group (P<0.05); ETS-1 and PKD1 mRNA levels were increased in the ETS-1 group (P<0.05). In contrast with the control group, the mRNA levels of autophagy markers including Atg5 (P<0.05) and Beclin1 (P<0.01) were obviously decreased in the shPKD1 group, but they were obviously increased in the ETS-1 group (P<0.001). Protein levels of Atg5, Beclin1 and LC3 were significantly decreased in the shPKD1 group (P<0.05), but they were increased obviously in the ETS-1 group (P<0.05) in contrast with the control group. Conclusion PKD1 gene is involved in MOVAS cells autophagy, low expression of PKD1 gene can inhibit autophagy and high expression of PKD1 promotes autophagy in vascular smooth muscle cells.
