Effects of arecoline on apoptosis of human periodontal ligament fibroblasts
10.12016/j.issn.2096-1456.2017.04.004
- Author:
WANG Huan-ling
1
;
ZHANG Jian-ping
2
Author Information
1. Lushan Mountain Road Community Health Service Center of Tianjin
2. Second Affiliated Hospital of Tianjin University of TCM
- Publication Type:Journal Article
- Keywords:
Arecoline;
Human periodontal ligament fibroblasts;
Apoptosis;
Inhibition;
Protein expression
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2017;25(4):223-226
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To observe the effects of arecoline on the related protein expressions in the apoptosis of cultured human periodontal ligament fibroblasts (hPDLFs), such as p-JNK, p-p53, and Bcl-2.
Methods :hPDLFs were isolated from human periodontal ligament tissues and expanded in vitro. hPDLFs were treated with different concentrations of arecoline (0, 20, 40 and 80 μg/mL) for 12 h. The proliferative activities were evaluated by MTT. The expressions of p-JNK, p-p53, and Bcl-2 were determined by Western blot.
Results:Arecoline inhibited cell proliferation and induced apoptosis protein. The protein level of Bcl-2 was decreased, while p-JNK and p-p53 were increased (P < 0.05). The protein expressions were in a concentration-dependent manner with arecoline.
Conclusion :It demonstrates arecoline might contribute to the apoptosis of hPDLFs, and could destroy periodontal tissues.
- Full text:槟榔碱对人牙周膜成纤维细胞凋亡的影响.pdf
