miR-223-3p regulates proliferation and apoptosis of hepatocellular carcinoma SMMC-7721 cells by targeting RAC1
10.3872/j.issn.1007-385x.2020.06.012
- VernacularTitle:miR-223-3p通过靶向RAC1调控肝细胞癌SMMC-7721细胞的增殖和 凋亡
- Author:
QI Xin
1
;
WANG Huizi
2
;
CHEN Xudong
3
;
ZHANG Tianqi
4
;
YU Xiaolin
5
Author Information
1. Department of Laboratory, School Clinic of Northeast Electric Power University, Jilin 132000, Jilin, China
2. 2a. Department of Laboratory
3. 2b. Department of Gastroenterology
4. 2c. Special Second Cardiovascular Department, Jilin Central Hospital, Jilin 132000, Jilin, China
5. 3. Experimental Teaching Center, Stomatological Hospital of Jilin University, Changchun 130000, Jilin, China
- Publication Type:Clinical Trial
- Keywords:
miR-223-3p;
Ras-related C3 botulinum toxin substrate 1 (RAC1);
hepatocellular carcinoma (HCC);
SMMC-7721 cell;
proliferation;
apoptosis
- From:
Chinese Journal of Cancer Biotherapy
2020;27(6):664-670
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective: To investigate the effects of miR-223-3p on the proliferation and apoptosis of hepatocellular carcinoma (HCC) cells by regulating Ras-related C3 botulinum toxin substrate 1 (RAC1) and its possible mechanism. Methods: Thirty pairs of HCC and corresponding para-cancer tissues resected in Jilin Central Hospital from August 2016 to August 2018 were collected for this study; in addition, human HCC cell lines SMMC-7721, BEL-7402, HepG2 and human normal hepatocyte QSG-7701 were also collected. The expression level of miR-223-3p in HCC tissue and cell lines was detected by qPCR. miR-223-3p mimics, miR-223-3p inhibitor and siRAC1 were transfected into SMMC-7221 cells, respectively. CCK-8 assay, Colony formation assay and Annexin V-FITC/PI staining Flow cytometry were used to detect the proliferation, clone formation and apoptosis of SMMC-7721 cells, respectively. The relationship between miR-223-3p and RAC1 was confirmed by Dual luciferase reporter gene assay. The protein level of RAC1 in SMMC-7721 cells was detected by Western blotting. Results: The expression of miR-223-3p in HCC tissues was significantly lower than that in paracaner tissues (P<0.01), and had significant correlation with pathological characteristics, such as tumor size, TNM stage, EdmondsonSteiner grade (all P<0.05 or P<0.01). miR-223-3p expression in HCC cell lines was significantly lower than that in QSG-7701 cells with the lowest expression in SMMC-7721 cells. Dual luciferase reporter gene assay confirmed that RAC1 was a target gene of miR-223-3p, and miR-223-3p negatively regulated RAC1 expression. Over-expression of miR-223-3p significantly inhibited the proliferation and colony formation (P<0.05 or P<0.01) of SMMC-7721 cells and promoted cell apoptosis (P<0.01). Contrarily, knockdown of miR-223-3p reversed the inhibitory effect of miR-223-3p mimics on cells. Conclusion: miR-223-3p over-expression inhibits proliferation and colony formation and promotes apoptosis of HCC cells, the mechanism of which may be related with its targeted down-regulation of RAC1.
- Full text:20200612.pdf
