Expression and clinical significance of lncRNATTN-AS1 in lung adenocarcinoma
10.3872/j.issn.1007-385x.2020.06.010
- VernacularTitle:长链非编码RNATTN-AS1在肺腺癌中的表达及其临床意义
- Author:
JIA Yunlong
1
;
LIU Yan
1
;
WANG Yu
1
;
LIU Tianxu
1
;
WANG Jiali
1
;
LIU Lihua
1
Author Information
1. Department of Tumor Immunotherapy, Fourth Hospital of Hebei Medical University, Shijiazhuang 050035, Hebei, China
- Publication Type:Clinical Trial
- Keywords:
lung adenocarcinoma;
long non-coding RNA(lncRNA);
titin antisense RNA1 (TTN-AS1);
prognosis;
biomarker
- From:
Chinese Journal of Cancer Biotherapy
2020;27(6):653-657
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective：To study the expression of long non-coding RNA(lncRNA) titin antisense RNA1 (TTN-AS1) in lung adenocarcinoma (LUAD) tissues, and explore its relationship with clinicopathologic characteristics and prognosis of LUAD patients. Methods: The TTN-AS1 expression in LUAD data set was analyzed using TCGAdatabase. 52 pairs of tumor tissues and matched para-carcinoma tissues from LUAD patients, who underwent surgical resection and were later pathologically conformed in Fourth Hospital of Hebei Medical University between Jan. 2014 and Jan. 2015, were used in this study. qPCR was performed to detect TTN-AS1 expression in the specimens. Then, the correlations between TTN-AS1 expression and clinicopathologic characteristics were analyzed. Survival analysis was used to determine the significance of TTN-AS1 expression for predicting the prognosis of LUAD patients. Results: TCGAdatabase analysis and qPCR results showed that TTN-AS1 expression in LUAD tissues was significantly higher than that in normal lung and para-carcinoma tissues (both P<0.01). TTN-AS1 expression in LUAD tissues was significantly correlated with the TNM stage and lymph node metastasis (P<0.05), but not correlated with gender, age, tumor invasion range (P>0.05). Kaplan-Meier univariate analysis result demonstrated that the patients with high TTN-AS1 expression had shorter post-operative disease-free survival (DFS) and overall survival (OS) than those patients with low TTN-AS1 expression (all P<0.01). Cox proportional hazard regression model result demonstrated that wider tumor invasion range, positive lymph node metastasis and high TTN-AS1 expression were significantly correlated with shorter postoperative DFS and OS (P<0.05). Conclusion: TTN-AS1 was highly expressed in LUAD tissues, and closely correlated with TNM stage and lymph node metastasis of LUAD patients (all P<0.05). High expression of TTN-AS1 is significantly correlated with shorter DFS and OS, indicating that TTN-AS1 may be a biomarker for predicting poor prognosis of LUAD patients.
- Full text:20200610.pdf
