Over-expression of miR-145-5p inhibits malignant biological behaviors of esophageal squamous cells carcinoma via down-regulating IGF1R
10.3872/j.issn.1007-385x.2020.06.007
- VernacularTitle:过表达miR-145-5p通过下调IGF1R抑制食管鳞状细胞TE-10细胞的恶 性生物学行为
- Author:
BING Zhongxing
1
;
CAO Lei
1
;
CAO Zhili
1
;
LIANG Naixin
1
Author Information
1. Department of Thoracic Surgery, Peking Union Medical College Hospital, Beijing 100005, China
- Publication Type:Journal Article
- Keywords:
esophageal squamous cells carcinoma(ESCC)；TE-10 cell;
miR-145-5p;
insulin-like growth factor 1 receptor (IGF1R);
proliferation;
invasion;
migration;
epithelial-mesenchymal transition(EMT)
- From:
Chinese Journal of Cancer Biotherapy
2020;27(6):634-639
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective: To explore the mechanism of miR-145-5p on malignant biological behaviors, such as pro-liferation, invasion, migration and epithelial-mesenchymal transition (EMT), of esophageal squamous cell carcinoma (ESCC) TE-10 cells. Methods: The expression of miR-145-5p in ESCC cell lines and normal cells was detected by PCR. Dual luciferase reporter gene assay was used to detect the targeted regulation between miR-145-5p and insulin-like growth factor 1 receptor (IGF1R). The expres-sions of IGF1R protein and EMT related proteins were detected by Western blotting. Transwell assay and CCK-8 assay were carried out to detect the effects of miR-145-5p/IGF1R axis on the proliferation, migration andinvasionofTE-10 cells. Results: miR-145-5p was down-regulated in ESCC cell lines with the lowest expression in TE-10 cells (P<0.01orP<0.05).Over-expression of miR-145-5p significantly inhibited proliferation, invasion, migration and EMT of TE-10 cells (P<0.01 or P<0.05). Dual luciferase reporter gene assay con-firmed that miR-145-5p targetedly down-regulated IGF1R expression (P<0.01). The restora-tion experiments further confirmed that simultaneous over-expression of miR-145-5p and IGF1R significantly attenuated the promotion effect of IGF1R on proliferation, invasion, migration and EMT of TE-10 cells (P<0.01 or P<0.05). Conclusions: Over-expression of miR-145-5p inhibits proliferation, invasion, migration and EMT of ESCC TE-10 cells by down-regulating IGF1R.
- Full text:20200607.pdf
