Inhibitory effect of silencing HMGB1 gene on growth of human epithelial ovarian cancer xenografts in nude mice
10.3872/j.issn.1007-385x.2020.06.006
- VernacularTitle:沉默HMGB1基因对裸鼠人上皮性卵巢癌移植瘤生长的抑制作用
- Author:
WU Meiqin
1
,
2
,
3
;
WANG Yong
4
;
ZHU Hongfei
5
,
6
;
SONG Xiaojie
1
,
2
,
3
;
LI Yuxia1
1
,
2
,
3
;
LIU Zhihui
1
,
2
,
3
;
ZHAO Shuyan
5
,
6
;
YUAN Jing
5
,
6
;
GONG Jingjing
5
,
6
;
LIANG Xing
5
,
6
;
CHEN Dandan
5
,
6
;
NING Xiangcheng
5
,
6
Author Information
1. Department of Obstetrics and Gynecology, Wuhan Children'
2. s Hospital &
3. Wuhan Maternal and Child Health Care Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430015, Hubei, China
4. Animal Laboratory Center, Wuhan University, Wuhan 430070, Hubei, China
5. .Hubei Hospital of Traditional Chinese Medicine &
6. Hubei Institute of Traditional Chinese Medicine, Wuhan 430070, Hubei, China
- Publication Type:Journal Article
- Keywords:
high mobility group box 1 (HMGB1);
ovarian cancer;
SKOV3 cell;
VEGF;
apoptosis
- From:
Chinese Journal of Cancer Biotherapy
2020;27(6):629-633
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective: To investigate the effect of HMGB1 gene on the growth of human epithelial ovarian cancer xenografts in nude mice, and to lay a foundation for finding new targets for the treatment of ovarian cancer. Methods: Human epithelial ovarian cancer SKOV3 cells in logarithmic growth phase were selected to establish a human epithelial ovarian cancer xenograft model in nude mice. Nude mice with successful model establishment were randomly divided into control group and HMGB1-siRNA group. On the 7th, 9th, 11th, 14th, and 16th days after cell inoculation, the same amount of saline and HMGB1-siRNA were respectively injected into two groups of mice under the armpit.After 3 weeks, the nude mice were sacrificed by cervical dislocation, the tumor tissues were separated, and the volume of the tumor was measured. The apoptosis of transplanted tumor cells was detected by Tunnel staining. The expressions of HMGB1, STAT3 and p-STAT3 were detected by Western blotting. The expression of vascular endothelial growth factorA(VEGF-A) and microvascularization were detected by immunohistochemistry. Results: Compared with the control group, the growth of tumor volume slowed down in HMGB1 siRNA group, and on the 21st day, the tumor volume of HMGB1-siRNA group was significantly smaller than that of the control group (P<0.05). HMGB1-siRNA successfully knocked down the expression of HMGB1 mRNA in transplanted
tumor tissue. The apoptosis rate of tissue cells in HMGB1-siRNA group was significantly increased ([34±8]% vs [6±2]%, P=0.04), and the expressions of HMGB1 and p-STAT3 were significantly reduced (P<0.05). The expression of VEGF-Aand the number of microvessels were significantly lower than those of the control group (both P<0.05). Conclusion: Knockdown of HMGB1 gene reduces the expression of VEGF-A and microvessel formation possibly by inhibiting the HMGB1/STAT3 signaling pathway, thereby promoting the apoptosis of tumor tissues and slowing the growth of xenografts.
- Full text:20200606.pdf
