Comparative Study on the Contents of Four Compounds from Different Processed Products of Miao Medicine Chimonanthus nitens and Its Toxicity
- VernacularTitle:苗药铁筷子不同炮制品中4种成分含量及毒性的比较研究
- Author:
Xue WANG
1
,
2
,
3
;
Li WANG
1
,
2
;
Xiu LIAO
1
,
2
;
Haibo WANG
4
;
Juan YANG
1
,
2
;
Qiji LI
1
,
2
;
Xiaosheng YANG
1
Author Information
1. State Key Laboratory for Efficacy and Utilization of Medicinal Plants，Guizhou Medical University，Guiyang 550014，China
2. Guizhou Provincial Key Laboratory for Natural Product Chemistry，Chinese Academy of Sciences，Guiyang 550014，China
3. College of Pharmacy，Guizhou Medical University，Guiyang 550016，China
4. Sunflower Pharmaceutical Gro up（Guizhou）Hongqi Co.，Ltd.，Guizhou Liupanshui 553400，China
- Publication Type:Journal Article
- Keywords:
Chimonanthus nitens;
Processing;
Content;
Acute toxicity;
Glycosides;
Mice
- From:
China Pharmacy
2020;31(12):1475-1462
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To compare the contents of 4 components and toxicity from Miao medicine Chimonanthus nitens samples after drying in the shade ，oven drying ，steam，charring. METHODS ：The contents of scopolin，fraxin，scopoletin and isofraxin were simultaneously determined by HPLC. The separation was performed on Welch-C18 column with mobile phase consisted of acetonitrile-water （gradient elution ）at the flow rate of 1 mL/min. The detection wavelength was set at 214 nm，and column temperature was 30 ℃，and the sample size was 10 μL. Modified Karber method was used to determine the LD 50 and its 95％CI of different processed C. nitens to mice and evaluate its acute toxicity. RESULTS ： The linear range of scopolin ， fraxin， scopoletin and isofraxin were 0.019-1.856，0.016-1.616，0.009-0.920，0.006-0.624 μg（R2 were all not lower than 0.999 5）. RSDs of precision ， stability and reprodu-cibility tests were all lower than 2.0％（n＝6）. The average recoveries were 104.49％，102.22％， 101.45％，99.26％（all RSDs were lower than 2％，n＝6）. The contents of scopolin were 1.119 0％，0.904 3％， 1.068 4％and 0.036 4％；those o f fraxin were 0.867 8％，0.453 9％，0.423 7％and 0.020 5％；those of scopoletin ； those of isofraxidin were 0.110 2％，0.202 1％，0.208 1％ and 0.249 4％in samples after drying in the shade ，oven CX-2018-001） drying， steam， charring. The LD 50 of 4 processedproducts were 4 118.13，3 733.36，1 643.61，＞10 000 qq.com mg/kg samples ；95％CI were （3 748.87，4 523.76）， （3 422.16，4 072.86），（1 520.90，1 776.23），（＞10 000） mg/kg. CONCLUSIONS ：HPLC method is reproducible and precise. It can be used to determine the contents of 4 components in different processed products of C. niten s. The contents of 4 components in different processed products ， and the contents of glycosides toxicity components are decreased significantly. All the 4 processed products were low or non-toxic.
