Study on Antitumor Mechanism of Periplaneta americana Extract C Ⅱ-3 on MFC Tumor-bearing Mice Based on 1H-NMR Metabonomics
- VernacularTitle:美洲大蠊提取物CⅡ-3对MFC荷瘤小鼠抑瘤机制的1H-NMR代谢组学研究
- Author:
Ying LI
1
;
Zhuping TAO
1
;
Xu CHANG
2
;
Hongli OU
2
,
3
;
Lun JIANG
2
,
4
;
Canwei LI
5
;
Yanlong TANG
6
;
Yue ZHOU
2
;
Li BAI
2
;
Pengfei GAO
1
Author Information
1. College of Pharmacy and Chemistry，Dali University，Yunnan Dali 671000，China
2. College of Basic Medical Sciences，Dali University，Yunnan Dali 671000，China
3. Dept. of Blood Transfusion，Dehong People’s Hospital，Yunnan Dehong 678400，China
4. Dept. Two of Internal Medicine，Yongxing County Traditional Chinese Medicine Hospital of Hunan Province，Hunan Chenzhou 423300，China
5. College of Public Health，Dali University，Yunnan Dali 671000，China
6. Dept. of Radiology，the First Affiliated Hospital of Dali University，Yunnan Dali 671000，China
- Publication Type:Journal Article
- Keywords:
Periplaneta americana extract C Ⅱ-3;
MFC cell line;
Metabolomics;
1H-NMR;
Mice
- From:
China Pharmacy
2020;31(12):1446-1451
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To preliminarily study the antitumor mechanism of Periplaneta americana extract C Ⅱ-3 on MFC tumor-bearing mice. METHODS ：Balb/c mice were randomly divided into model group （normal saline 20 mL/kg）and C Ⅱ-3 group （200 mg/kg），with 6 mice in each group. MFC cell suspension （0.2 mL）was injected under the right armpit of mice. On the next day，mice were given relevant medicine intragastrically ，once a day ，for consecutive 10 d. 24 h after the last administration ，Based on the measurement of tumor size ， 1H-NMR technology combined with unsupervised PCA ，supervised PLS-DA and OPLS-DA were used to compare metabolic spectrum of liver tissue from tumor-bearing mice of 2 groups，to analyze differential metabolites and to explore the potential antitumor mechanis m of C Ⅱ -3. RESULTS ：Compared with model group ，the tumor body was significantly reduced in tumor-bearing mice of C Ⅱ-3 group. There were differences in 1H-NMR spectra between the 2 No.81960712）； groups. According to unsupervised PCA ，supervised PLS-DA and OPLS-DA ，totally six potential differential metabolites ，as glycogen （increased），pyruvate （decreased），arginine （de- creased），hydroxyproline （increased），inosine （increased） and niacinamide （increased），were identified in the liver tissue，which were mainly attributed to the metabolism of arginine ，energy and nucleic acid. CONCLUSIONS：The anti tumor effect of C Ⅱ-3 may be related to the regulation of arginine metabolism ，energy metabolism and nucleic acid metabolism.
