Study on the Active Ingredients and Mechanism of Alpinia officinarum in the Treatment of Gastric Ulcer Based on Network Pharmacology
- VernacularTitle:基于网络药理学探讨高良姜治疗胃溃疡的活性成分及作用机制
- Author:
Zhenmiao QIN
1
;
Lili ZHANG
2
;
Demei CHEN
1
;
Ning WANG
1
;
Yingzi CHEN
1
;
Xinfu WU
1
;
Junqing ZHANG
1
Author Information
1. School of Pharmacy，Hainan Medical University，Haikou 571199，China
2. Dept. of Cardiovasology，Hainan Provincial People’s Hospital/Hainan Hos pital Affiliated Hainan Medical University，Haikou 570311，China
- Publication Type:Journal Article
- Keywords:
Network pharmacology;
Alpinia officinarum;
Gastric ulcer;
Active ingredients;
Mechanism
- From:
China Pharmacy
2020;31(12):1440-1439
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To explore the potential active ingredients and mechanism of Alpinia officinarum in the treatment of gastric ulcer. METHODS ：By network pharmacology method ，the active ingredients and action targets of A. officinarum were screened through TCMSP and TCMID database retrieval [oral bioavailability （OB）≥30％ and drug like （DL）≥0.18] and literature mining. Targets of gastric ulcer were obtained in the TTD ，CTD，OMIM，PubMed，DrugBank and DisGeNet databases. Venny 2.1 software was used to screen common targets for the active ingredients of A. officinarum and gastric ulcer. Then ，the protein-protein interaction（PPI）of the common targets was obtained by STRING database ，and the PPI network was constructed and analysed by using Cytoscape 3.7.1 software. GO function and KEGG pathway enrichment analysis of the common targets were performed by using ClusterProfiler R package. Finally ，Cytoscape 3.7.1 software was used to construct and analyze the network diagram of “active ingredients-targets-pathways ”. RESULTS ：Totally 19 active ingredients of A. officinarum ，209 active ingredients targets and 195 gastric ulcer related targets ，involving 35 common targets ，were screened out. The average node degree of PPI network of common targets was 18，and the average intermediate number was 16.9. There were 11 key targets ，i.e. PTGS2，VEGFA，IL6， IL1B，CCL2，MYC，MMP9，EGFR，HIF1A，ESR1，BCL2L1. The common targets were mainly concentrated in the cell constituents such as the platelet α granule lumen and mitochondria outer membrane ，involved in the biological processes as oxidative stress ，inflammatory response regulation ，and molecular functions as protein phosphatase binding ，growth factor receptor binding. They were also enriched in the signal pathways such as PI3K/Akt，HIF-1. The network of“active ingredients- targets-pathways”showed the active ingredients such as quer- cetin，apigenin，kaempferol and galangin in A. officinarum played an anti-gastric ulcer effect by acting on PTGS2，NOS2， BCL2， IL6， VEGFA， IL1B， MMP9， BCL2L1 and other targets to jointly regulate PI 3K-Akt，HIF-1，TNF，IL-17， NF-κB and other cell proliferation，angiogenesis，and infla- 163.com mmation related pathways. CONCLUSIONS ：A. officinarum shows anti-gastric ulcer effect with the characteristics of multi-ingredient ，multi-target and multi-path.
