Apoptotic Effects of Genistein, Biochanin-A and Apigenin on LNCaP and PC-3 Cells by p21 through Transcriptional Inhibition of Polo-like Kinase-1.
10.3346/jkms.2011.26.11.1489
- Author:
Young Jin SEO
1
;
Bum Soo KIM
;
So Young CHUN
;
Yoon Kyu PARK
;
Ku Seong KANG
;
Tae Gyun KWON
Author Information
1. Department of Urology, College of Medicine, Dongguk University, Gyeongju, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Prostatic Neoplasms;
Apoptosis;
Flavones;
Isoflavones
- MeSH:
Antineoplastic Agents/*pharmacology;
Apigenin/pharmacology;
*Apoptosis;
Cell Cycle/drug effects;
Cell Cycle Proteins/biosynthesis/*genetics/metabolism;
Cell Line, Tumor;
Cell Proliferation;
Cyclin-Dependent Kinase Inhibitor p21/biosynthesis/*metabolism;
Flavonoids/*pharmacology;
Gene Expression Regulation, Neoplastic/drug effects;
Genistein/pharmacology;
Humans;
Male;
Prostatic Neoplasms/genetics/metabolism/*pathology;
Protein Kinase Inhibitors/pharmacology;
Protein-Serine-Threonine Kinases/biosynthesis/*genetics/metabolism;
Proto-Oncogene Proteins/biosynthesis/*genetics/metabolism;
Transcription, Genetic/drug effects
- From:Journal of Korean Medical Science
2011;26(11):1489-1494
- CountryRepublic of Korea
- Language:English
-
Abstract:
Natural isoflavones and flavones are important dietary factors for prostate cancer prevention. We investigated the molecular mechanism of these compounds (genistein, biochanin-A and apigenin) in PC-3 (hormone-independent/p53 mutant type) and LNCaP (hormone-dependent/p53 wild type) prostate cancer cells. A cell growth rate and apoptotic activities were analyzed in different concentrations and exposure time to evaluate the antitumor activities of genistein, biochanin-A and apigenin. The real time PCR and Western blot analysis were performed to investigate whether the molecular mechanism of these compounds are involving the p21 and PLK-1 pathway. Apoptosis of prostate cancer cells was associated with p21 up-regulation and PLK-1 suppression. Exposure of genistein, biochanin-A and apigenin on LNCaP and PC-3 prostate cancer cells resulted in same pattern of cell cycle arrest and apoptosis. The inhibition effect for cell proliferation was slightly greater in LNCaP than PC-3 cells. In conclusion, flavonoids treatment induces up-regulation of p21 expression, and p21 inhibits transcription of PLK-1, which promotes apoptosis of cancer cells.
