Role of TRAF6 in inflammatory responses of human osteoblast-like cells with Enterococcusfaecalis
10.12016/j.issn.2096-1456.2017.07.003
- Author:
LU Yu
1
;
LIU Chengxia
1
;
LIU Zhongjun
1
Author Information
1. Department of Endodontics, Stomatological Hospital, Southern Medical University
- Publication Type:Journal Article
- Keywords:
E. faecalis;
LTA;
TLR2;
Human osteoblast-like cells;
TRAF6
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2017;25(7):420-425
- CountryChina
- Language:Chinese
-
Abstract:
Objective :Explore the role and status of tumor necrosis factor receptor-associated factor 6 (TRAF6) in the inflammatory response of human osteoblast-like cells MG63 which was triggered by Enterococcus faecalis (E. faecalis) and its lipoteichoic acid (LTA)
Methods: SiRNA technology was applied to silence the TRAF6 gene of MG63 cells, Using E.faecelis and its LTA to stimulate the silence MG63 cells with different hours. After that, using real-time PCR technology to detect toll-like receptor 2 (TLR2) and TRAF6 gene expression and using ELISA assay to detect proinflammatory cytokines interleukin-1β and TNF-alpha expression levels.
Results:When MG63 cells was infected by E. faecalis, its LTA, TLR2 and TRAF6 gene level has increased to varying degrees (P< 0.05); interleukin-1β and TNF-alpha expression was significantly higher (P< 0.05). When TRAF6 gene of MG63 cells was silenced by siRNA, pro-inflammatory cytokines interleukin-1β, interleukin-6, interleukin -8 and TNF-alpha expression decreased significantly (P< 0.05).
Conclusion:E. faecalis and its toxic components is identified by MG63 cells mainly through TLR2 receptors. The major virulence factor in periapical infections caused by E. faecalis is LTA.
- Full text:TRAF6在粪肠球菌感染人成骨样细胞炎症反应中的作用.pdf
