Effect of Notch signaling and autophagy on MTA induced differentiation of human dental pulp cells in vitro
10.12016/j.issn.2096-1456.2017.07.002
- VernacularTitle:Notch信号及自噬在三氧化矿化聚合物促人牙髓细胞体外分化中的作用
- Author:
HE Fei
1
,
2
,
3
,
4
;
ZHENG Yuyan
1
,
2
,
3
,
4
;
QIU Wei
5
;
ZHANG Guoquan
1
,
2
,
3
,
4
;
MAI Sui
6
Author Information
1. Department of Stomatology, the Second Clinical Medical College &
2. Shenzhen People'
3. s Hospital, Ji'
4. nan University
5. Key Lab in Healthy Science and Technology, Division of Life Science, Graduate School at Shenzhen, Tsinghua University
6. Department of Conservative Dentistry and Endodontics Guanghua School of Stomatology, Sun Yat-sen University
- Publication Type:Journal Article
- Keywords:
Mineral trioxide aggregate;
Dental pulp cells (DPCs);
Differentiation;
Notch;
Autophagy
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2017;25(7):414-419
- CountryChina
- Language:Chinese
-
Abstract:
Objective: The aim of this study is to investigate the roles of Notch signaling and autophagy on mineral trioxide aggregate (MTA) induced differentiation of human dental pulp cells (hDPCs).
Methods :Third molars from healthy human were collected and hDPCs were isolated by a combined digestion of collagenase Ⅰ and dispaseⅡ. Real time PCR were used to test the mRNA expression levels of alkaline phosphatase (ALP), runt-related transcription factor 2 (Runx2) and dentin sialophoprotein (DSPP) in MTA treated hDPCs in different time (24 h, 3 d and 7 d). The mineralization nodules formed by hDPCs with or without MTA treatment were detected by Von Kossa staining. Expressions of Notch1, Jagged1, Hes1, LC3Ⅱ/LC3 Ⅰand p62 in wild type and MTA treated hDPCs were detected by western blotting.
Results:MTA extracted in a concentration of 0.1 mg/mL could promote the differentiation of hDPCs. Compared with that of wild type hDPCs, the expressions of Notch1, Hes1, or Jagged1 and p62 (P<0.01) in MTA treated hDPCs were significantly increased. MTA treatment showed inhibition effects on autophagy flux similar to Bafilomycin A1, a specific inhibitor of fusion between autophagosomes and lysosomes.
Conclusion:MTA could promote hDPCs differentiation with highly relevant in stimulating Notch1-Jagged1-Hes1 signaling and inhibition of autophagy flux.
- Full text:Notch信号及自噬在三氧化矿化聚合物促人牙髓细胞体外分化中的作用.pdf
