CXCR4/SDF-1 axis regulates the effect of human lung adenocarcinoma PC-9 cells on function of in vitro blood-brain barrier model formed by Bends cells
10.3872/j.issn.1007-385x.2020.05.009
- VernacularTitle:CXCR4/SDF-1轴调节人肺腺癌PC-9细胞对Bends细胞体外血脑屏障模 型功能的影响
- Author:
LI Hongru
1
,
2
;
TU Xunwei
1
,
2
;
CHEN Zhengwei
1
,
2
;
CHEN Yusheng
1
,
2
;
HAN Lili
3
Author Information
1. (a. Department of Respiratory and Critical Medicine
2. b. Fujian Institute of Respiratory Diseases
3. c. Fujian Key Laboratory of Cardiovascular Diseases, Fujian Provincial Hospital, Provincial Clinical Medical College of Fujian Medical University, Fuzhou 350001, Fujian, China
- Publication Type:Journal Article
- Keywords:
lung adenocarcinoma;
PC-9 cell;
blood brain barrier (BBB);
Bends cell;
tight junction;
transendothelial electric resistance (TEER);
migration;
CXCR4/SDF-1 axis
- From:
Chinese Journal of Cancer Biotherapy
2020;27(5):528-533
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective: To investigate the influences of human lung adenocarcinoma PC-9 cells on tight junction proteins of blood brain barrier (BBB) under CXCR4/SDF-1 axis by establishing a model of BBB in vitro. Methods: The immortalized mouse brain microvascular endothelial Bends cells were used to establish a model of BBB in vitro by monolayer culture; Subsequently, transendothelial electric resistance (TEER) and fluorescein sodium permeability experiment were used to detect the function of in vitro BBB model and observe the effect of PC-9 cells on the function of BBB model, respectively. Western blotting was used to detect the effect of PC-9 cells on function of BBB model and expressions of endothelial tight junction proteins under the treatment of single or combined AMD3100 and SDF-1 (1 μg/ml AMD3100,100 ng/ml SDF-1, AMD3100+SDF-1). Transwell assay was used to detect the influence of CXCR4/SDF-1 axis on the ability of PC-9 cells transmigrating the cell layer of BBB model. Results: Monolayer culture of Bends cells can form tightly connected BBB withhighTEER,which reached (182.13±5.19) Ω.cm2 at the 96 h; in the meanwhile, fluorescein sodium permeability experiment showed that BBB had significantly lower permeability than that of control group ([40.31±2.43]% vs [150.10±3.17]%, P<0.05). The TEER of BBB decreased to (46.7±4.35) Ω·cm2 after coculture with PC-9 cells for 24 h, and at the same time the sodium fluorescein permeability of BBB significantly increased than that of pre-treatment ([136.32±4.93]% vs [50.24±6.21]%, P<0.05). PC-9 cells up-regulated the expressions of tight junction proteins of Bends cells under the treatment of AMD3100 (P<0.05). The number of PC-9 cells transmigrating the BBB inAMD3100 treatment group was significantly lower than that of CON group (43±2 vs 81±2, P<0.05). Conclusion: AMD3100 can reduce the ability of PC-9 cells destroying the tight junction of the BBB model established in vitro by Bends cells.
- Full text:20200509.pdf
