Effect of midkine on expression of non-coding RNA in ovarian cancer
10.13602/j.cnki.jcls.2019.12.05
- VernacularTitle:中肾因子Midkine对卵巢癌中非编码RNA表达的影响
- Author:
Meina JIANG
1
;
Ting YUAN
2
;
Jingyun ZHANG
2
;
Jingyan XIE
2
;
Shuli ZHAO
3
Author Information
1. Clinical Nuclear Medicine Center, Nanjing Medical University
2. Department of Gynecology, Nanjing Medical University
3. Central Laboratory, Nanjing First Hospital, Nanjing Medical University
- Publication Type:Journal Article
- Keywords:
midkine;
ovarian cancer;
long chain non-coding RNA;
single chain microRNA;
gene chip
- From:
Chinese Journal of Clinical Laboratory Science
2019;37(12):897-900
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the correlation of non-coding RNA and the tumor-associated antigen midkine (MK) in SKOV3cells and the clinical significance for diagnosis of ovarian cancer.
Methods:The Agilent′s gene chips (miRNAs chip and lncRNAs chip) were used to analyze the differential expression of miRNAs and lncRNAs in both MK-overexpressing SKOV3-MK cells and the control SKOV3-Con cells to screen the potential biomarkers in ovarian cancer. The clinical significance of midkine in the serum and tissues samples was analyzed for the patients with ovarian cancer by quantitative PCR combined with clinical data.
Results:Compared with control SKOV3-con cells, MK overexpression significantly promoted the expressions of 11 miRNAs and 7 lncRNAs in SKOV3 cells (P＜0.01, ratio＞3 fold), reduced the expressions of 8 miRNAs and 13 lncRNAs (P＜0.01, ratio＜0.3). Results of qPCR showed that the expression level of miR489 was significantly lower in ovarian cancer tissues than that of the contralateral normal ovarian tissues, while HOTAIR was significantly elevated (P＜0.05). The expression level of HOTAIR in the serum of ovarian cancer patients was significantly higher than that in healthy controls group with same age (0.036±0.024 vs 0.019±0.020, P=0.002). ROC curve analysis of HOTAIR showed that the specificity was 66.7%, the sensitivity was 75.6% and the AUC value was 0.749 as a marker for serum detection of ovarian cancer when the cutoff value was 0.017 6.
Conclusion:Long-chain non-coding RNA HOTAIR may be served as a potential biomarker in serum of ovarian cancer patients.
