lncRNA HOXA-AS2 regulates proliferation, migration and invasion of ovarian cancer cells via targeting miR-520a-3p
10.3872/j.issn.1007-385x.2020.05.005
- VernacularTitle:lncRNAHOXA-AS2靶向miR-520a-3p调控卵巢癌细胞增殖、迁移和侵袭
- Author:
XIE Deling
1
,
2
;
LING Liefeng
3
;
JIN Xun
4
Author Information
1. (1. Department of Gynecology, The Second People'
2. s Hospital of Wuhu City, Wuhu 241001, Anhui, China
3. 2. Department of Biochemistry and Molecular Biology, Wannan Medical College, Wuhu 241002, Anhui, China
4. 3. Department of Chinese Medicine Surgery, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing 210029, Jiangsu, China
- Publication Type:Journal Article
- Keywords:
lncRNAHOXA-AS2;
miR-520a-3p;
ovarian cancer;
SKOV3 cell;
proliferation;
migration;
invasion
- From:
Chinese Journal of Cancer Biotherapy
2020;27(5):501-507
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective:To explore the targeting relationship between long-chain noncoding RNA HOXA-AS2 (lncRNA HOXA-AS2) and microRNA-520a-3p (miR-520a-3p) and their effects on the proliferation, migration and invasion of ovarian cancer SKOV3 cells. Methods： ：qPCR was used to detect the expression levels of lncRNA HOXA-AS2 and miR-520a-3p in various ovarian cancer cell lines (SKOV3, HO8910, OVCAR3 cells) and normal ovarian epithelial cell line HOSE. Bioinformatics methods were used to predict the targeting relationship between HOXA-AS2 and miR-520a-3p, which was then verified by Dual luciferase reporter gene assay. si-HOXA-AS2, miR-520a-3p mimic, anti-miR-520a-3p and corresponding control fragments were transfected into SKOV3 cells separately or in combination. MTT, Transwell and Western blotting were used to detect the proliferation, migration, invasion and expressions of related proteins (CyclinD1, p21, p27, MMP-2, MMP-9, MMP-14) of SKOV3 cells in each group. Results: Compared with HOSE cells, HOXA-AS2 was over-expressed while miR-520a-3p was under-expressed in ovarian cancer cell lines (all P<0.05). HOXA-AS2 could targetedly down-regulate the expression of miR-520a-3p. Compared with the NC group, the proliferation, migration and invasion of SKOV3 cells in the si-HOXA-AS2 and miR-520a-3p mimics groups were significantly reduced (all P<0.01), and the protein expressions of p21 and p27 were significantly increased, while protein expressions of CyclinD1, MMP-2, MMP-9, MMP-14 were significantly reduced (all P<0.01). The proliferation, migration and invasion of SKOV3 cells in the si-HOXA-AS2+antimiR-520a-3p group were significantly enhanced compared with those in si-HOXA-AS2 and si-HOXA-AS2+anti-miR-NC groups (all P<0.05). Conclusion: lncRNA HOXA-AS2 enhances the proliferation, migration and invasion of ovarian cancer SKOV3 cells by targetedly inhibiting the expression of miR-520a-3p.
- Full text:20200505.pdf
