Evaluation of different laboratory methods for auxiliary diagnosis of pulmonary tuberculosis
10.13602/j.cnki.jcls.2019.10.17
- VernacularTitle:不同实验室方法在肺结核辅助诊断中的应用评价
- Author:
Yiting YAO
1
,
2
;
Weihong XU
3
;
Meiyu TAN
3
;
Bin XU
3
;
Huiming SHENG
3
;
Ningli LI
1
Author Information
1. Shanghai Jiao Tong University School of Medicine
2. Department of Laboratory Medicine，Tong Ren Hospital，Shanghai Jiao Tong University School of Medicine
3. Department of Laboratory Medicine，Tong Ren Hospital，Shanghai Jiao Tong University School of Medicine
- Publication Type:Journal Article
- Keywords:
pulmonary tuberculosis；MGIT liquid culture；Xpert MTB/RIF
- From:
Chinese Journal of Clinical Laboratory Science
2019;37(10):789-792
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effectiveness of different laboratory methods for the supplementary diagnosis of pulmonary tuberculosis（PTB）and provide reference data for the early diagnosis of PTB.
Methods:A total of 298 suspected PTB patients, who were diagnosed and treated in the outpatient department of Shanghai Tongren Hospital from January 2016 to December 2018, were divided into 3 groups: active PTB (138 cases)，inactive PTB (43 cases) and non-PTB (117 cases) group. Sputum acid-fast staining, MGIT liquid culture system and Xpert MTB/RIF test were performed to detect the sputum specimens. The sensitivity and specificity were compared by Chi-square test.
Results:The three methods showed certain significance for distinguishing active PTB, inactive PTB combined with non-PTB (χ 2 values were 89.08, 138.94 and 137.12 respectively, all P＜0.01). There was no significant difference for the positive rate of the three methods between inactive PTB and non-PTB. The sensitivities of acid-fast staining, MGIT liquid culture, Xpert MTB/RIF test and the combination of three methods in the diagnosis of active PTB were 45.7% (63/138), 63.8% (88/138), 65.4% (87/133) and 78.2% (104/133) respectively. The sensitivities of MGIT culture and Xpert MTB/RIF test were significantly higher than that of acid-fast staining (χ 2 was 35.79 and 11.26 respectively，all P＜0.01). There was no significant difference for the sensitivities between MGIT liquid culture and Xpert MTB/RIF test（χ 2 was 29.87, P＞0.05) . The sensitivity of combined detection was higher than that of single detection（χ 2 was 30.84, 64.62, 70.14, respectively, all P＜0.01）. The diagnostic specificities of the three methods and their combination were 99.1%（116/117), 98.3%（115/116）, 99.1%（113/114) and 97.3%（110/113）respectively. There was no significant difference for the specificities of the three methods.
Conclusion:High sensitivities of MGIT liquid culture and Xpert MTB/RIF test were shown in PTB diagnosis. Combined detection of the three methods may improve the sensitivity of detection.
