Ovarian cancer cells promote glycolysis metabolism of peripheral CD4 + T cells
10.13602/j.cnki.jcls.2019.10.02
- VernacularTitle:卵巢癌细胞促进外周血CD4+T细胞糖酵解代谢
- Author:
Wenwen SHANG
1
;
Rui XU
1
;
Ming WU
1
;
Litao ZHANG
1
;
Xiaojie ZHANG
1
;
Fang WANG
1
Author Information
1. Department of Laboratory Medicine, the First Affiliated Hospital of Nanjing Medical University
- Publication Type:Journal Article
- Keywords:
ovarian cancer;
CD4 + T cell;
glucose metabolism
- From:
Chinese Journal of Clinical Laboratory Science
2019;37(10):726-731
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of ovarian cancer cells on the glycolysis metabolism and functions of peripheral CD4 + T cells.
Methods:We established a coculture system of human CD4 + T cells with ovarian cancer cell SKOV3, and the mRNA expression levels of 8 glycolysis-related genes (mTORC1, HIF1α, GLUT1, GPI, ENO1, PKM2, LDHα and PDK1) in cocultured CD4 + T cells were determined by real-time fluorescence quantitative PCR. The levels of glucose uptake and glycolysis were detected by colorimetry. The proportion of CD4 + CD25 + Foxp3 + T cells was tested by flow cytometry. The proliferation levels of CD4 + T cells were measured by tritium incorporation method. The expression levels of 8 glycolysis-related genes in peripheral CD4 + T cells were determined by real-time fluorescence quantitative PCR in 10 ovarian cancer (OC) patients, 10 benign ovarian tumor (BOT) patients and 10 healthy control (HC).
Results:Results: Compared with the CD4 + T cells in single culture without SKOV3, the expression levels of 8 glycolysis-related genes in CD4 + T cells cocultured with SKOV3 were elevated (all P＜0.01). The levels of glucose uptake and glycolysis were increased (P＜0.05). The proportion of CD4 + CD25 + Foxp3 + T cells was up-regulated (P＜0.05) and the proliferation levels of CD4 + T cells were decreased (P＜0.05). Moreover, the mRNA expression levels of all the glycolysis-related genes were increased (all P＜0.05) in the peripheral CD4 + T cells of OC patients compared with BOT patients and HC.
Conclusion:Ovarian cancer cells could promote the glycolysis metabolism of CD4 + T cells, which may be relevant to its differentiation and functions.
