Circulation miR-193 cluster serves as a promising biomarker for diagnosis of breast cancer
10.13602/j.cnki.jcls.2019.08.04
- VernacularTitle:循环microRNA-193簇对乳腺癌的筛查价值
- Author:
Bei PAN
1
;
Xuhong WANG
2
;
Shukui WANG
1
;
Bangshun HE
1
Author Information
1. General Clinical Research Center, Nanjing First Hospital, Nanjing Medical University
2. School of Medicine, Southeast University
- Publication Type:Journal Article
- Keywords:
microRNA;
breast cancer;
biomarker;
diagnosis
- From:
Chinese Journal of Clinical Laboratory Science
2019;37(8):574-578
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate circulating miR-193 cluster as a biomarker for diagnosis of breast cancer (BC).
Methods:A total of 130 BC patients and 45 healthy controls (HCs) were enrolled. The level of miR-193 cluster was analyzed by using GEO database. RT-qPCR was used to evaluate the level of miR-193 cluster in serum of participants. Receivers operating characteristic (ROC) curve analysis was manipulated to investigate their diagnostic value for BC.
Results:In the screening stage, the results of GEO database indicated the expression of miR-193a-5p and miR-193b were decreased in the serum of BC patients with prior diagnostic value, and the significant decreased expressions of miR-193 cluster members, miR-193a-3p, -5p and miR-193b-3p, were observed in serum of BC patients compared with those in healthy controls by the determination of RT-qPCR（all P ＜0.01）. In the validation stage, the AUCROC of miR-193a-5p and miR-193b-5p were 0.888 (95% CI :0.808-0.969) and 0.954 (95% CI :0.902-1.000), respectively. In addition, the combined AUCROC of these two miRNAs was 0.982 (95% CI :0.966-0.999).
Conclusion:miR-193a-5p and miR-193b-5p in serum could be served as diagnostic biomarker for screening of breast cancer, and the combined detections of the two miRNAs may exhibit more diagnostic efficiency and good clinical application potential.
