Expression of hsa_circ_0128298 in hepatocellular carcinoma and its functional roles in tumor growth
10.13602/j.cnki.jcls.2019.08.03
- VernacularTitle:Hsa_circ_0128298在肝癌中的表达及其参与肿瘤生长的功能
- Author:
Yi LI
1
,
2
;
Mingzhu KONG
3
;
Baihui ZHU
3
;
Lin CHEN
4
;
Weihua CAI
4
;
Shaoqing JU
1
,
2
;
Feng WANG
1
,
2
Author Information
1. Department of Laboratory Medicine, Affiliated Hospital of Nantong University
2. Department of Laboratory Medicine, School of Public Health, Nantong University
3. Department of Laboratory Medicine, School of Public Health, Nantong University
4. Department of Laboratory Medicine, Nantong Third Hospital Affiliated to Nantong University
- Publication Type:Journal Article
- Keywords:
hepatocellular carcinoma;
circular RNA;
hsa_circ_0128298;
p21
- From:
Chinese Journal of Clinical Laboratory Science
2019;37(8):568-573
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of circular RNA hsa_circ_0128298 in hepatocellular harcinoma (HCC) and evaluate its function in the growth process of HCC.
Methods:qPCR was used to detect the expressions of hsa_circ_0128298 in 50 HCC tissues, corresponding paracancerous tissues and HCC cells. The effects of hsa_circ_0128298 on HCC growth was analyzed by CCK-8 kit, flow cytometry and western blot assays. The values of hsa_circ_0128298 for the diagnosis and prognosis of HCC were assessed by ROC curve and survival curve analyses.
Results:Compared with the paracancerous tissues, the expression of hsa_circ_0128298 was significantly increased in HCC tissues ( U =846.0， P =0.005). The area under ROC curve (AUCROC) was 0.661, 95% confidence interval ( CI ) was 0.560 to 0.753, sensitivity was 80.0% and specificity was 50.0%. HCC patients with high expression of hsa_circ_0128298 displayed less overall survival time than those with low expression of hsa_circ_0128298 ( χ 2=6.294, P =0.012). RNA interference of hsa_circ_0128298 (si-hsa_circ_0128298) significantly inhibited HCC cell proliferation and induced cell cycle arrest at G0/G1, and also induced mRNA upregulation and protein expression of cyclin p21. Meanwhile，si-p21 partially reversed the proliferation inhibition and cell cycle arrest of HCC cells induced by si-hsa_circ_0128298, and restore its growth and proliferation potential.
Conclusion:The highly expressed hsa_circ_0128298 in HCC could promote the growth of HCC by regulating the expression of p21 and promise to be a new target for diagnosis and treatment of HCC.
