Differential expression analysis of circRNA in peripheral blood of tuberculosis patients in Xinjiang
10.13602/j.cnki.jcls.2019.07.06
- VernacularTitle:耐万古霉素肠球菌基因分型及耐药性分析
- Author:
Chen CHENG
1
;
Zhiwei TAN
1
;
Yingmei LIU
2
;
Shuai ZONG
3
;
Fei JIANG
3
;
Shuoshuo XIE
1
;
Bing GU
1
,
4
Author Information
1. School of Medical Technology, Xuzhou Medical University
2. Clinical Microbiology and Infection Laboratory, ChinaJapan Friendship Hospital
3. Clinical Laboratory, the Affiliated Hospital of Xuzhou Medical University
4. Clinical Laboratory, the Affiliated Hospital of Xuzhou Medical University
- Publication Type:Journal Article
- Keywords:
tuberculosis;
circRNA;
gene chip;
target gene
- From:
Chinese Journal of Clinical Laboratory Science
2019;37(7):504-507
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To find the differential expression profiles of circRNA in whole blood and predict its target genes in blood of patients with tuberculosis in Xinjiang, and explore the relationship between circRNA and the development of tuberculosis.
Methods:The circRNAs expression in peripheral blood from 3 pulmonary tuberculosis patients and 3 healthy individuals were tested by using circRNA microarray assay. The whole blood from 43 patients with tuberculosis, 40 healthy individuals and 43 patients with pneumonia were collected to verify the results by real-time quantitative PCR system. The possibility of differentially expressed circRNA target genes were predicted by circRNA target gene prediction database.
Results:In the results of microarray assay 835 circRNAs were found to be expressed differentially in whole blood between the tuberculosis patients and healthy controls of Xinjiang area, of which 249 circRNAs were up-regulated and 586 circRNAs were down-regulated in the patients. The expressions of four significantly different circRNA were verified by real time quantitative PCR and the results showed that hsa_circ_0008276, hsa_circ_0003452, hsa_circ_0001846 and hsa_circ_0090508 were down-regulated (P＜0.05), and hsa_circ_0090508 was the more specific than the other three circRNAs. The results of circRNA target genes prediction suggested that has-miR-1294, has-miR-604, has-miR-616, has-miR-663b and has-miR-486-3p may be the potential target genes of hsa_circ_0090508.
Conclusion:The differentially expressed circRNA hsa_circ_0090508 was significantly downregulated in the patients with tuberculosis and may affect the regulation mechanism of tuberculosis through target genes.
