β2GP/anti-β2GP complex inhibits oxLDL-mediated lipid accumulation and FAK activation in THP-1 macrophages
10.13602/j.cnki.jcls.2019.06.01
- VernacularTitle:β2GP/抗β2GP抗体复合物抑制oxLDL介导的THP-1巨噬细胞脂质蓄积和FAK活化
- Author:
Chao He
1
;
Hong ZHOU
1
;
Guiting ZHANG
1
;
Yudan CHEN
1
;
Peng ZHANG
1
;
Ren WANG
1
;
Qianqian WU
1
;
Yuye YAO
1
;
Ming KUANG
1
Author Information
1. School of Medicine，Jiangsu University
- Publication Type:Journal Article
- Keywords:
β2 glycoprotein Ⅰ/anti-β2 glycoprotein Ⅰ complex；oxidized low density lipoprotein；Toll-like receptor 4；lipid transportation；focal adhesion kinase；THP-1 macrophage
- From:
Chinese Journal of Clinical Laboratory Science
2019;37(6):401-406
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of β2 glycoprotein Ⅰ/anti-β2 glycoprotein Ⅰ complex (β2/aβ2) on oxidized low density lipoprotein (oxLDL)-mediated lipid accumulation and focal adhesion kinase (FAK) activation in THP-1 macrophage, as well as the role of Toll-like receptor 4 (TLR4) during the process.
Methods:THP-1 cells were differentiated into THP-1 macrophage by PMA (100 ng/mL). THP-1 macrophages were treated with RPMI 1640 medium, oxLDL, oxLDL+β2/aβ2 or oxLDL+lipopolysaccharide (LPS). The mRNA expressions of lipid transportation molecules, ACAT1, ABCA1 and ABCG1 were detected by RT-qPCR. Intracellular total cholesterol (TC) and free cholesterol (FC) in THP-1 macrophages were evaluated by Trinder assay, then the content and proportion of intracellular cholesteryl ester (CE) were calculated. The expression and phosphorylation of FAK were detected by immune fluorescence, RT-qPCR and western blot. To evaluate the role of TLR4, THP-1 macrophages were pre-treated with or without TLR4 inhibitor TAK-242 (1 μg/mL).
Results:β2/aβ2 treatment significantly inhibited oxLDL-mediated lipid accumulation and FAK expression and phosphorylation in THP-1 macrophages, which could be reversed by TLR4 blockage.
Conclusion:β2/aβ2 inhibits the oxLDL-mediated lipid accumulation and FAK activation of THP-1 macrophage, which is related to the function of TLR4.
