Effects of lncRNA LINC00473 on proliferation and migration of human gastric cancer cells
10.13602/j.cnki.jcls.2019.05.16
- VernacularTitle:长链非编码RNA LINC00473对人胃癌细胞增殖和迁移能力的影响
- Author:
Hong ZHU
1
;
Wei ZHANG
1
;
Guoqian LIU
1
;
Hongxia MENG
2
;
Shihe SHAO
1
Author Information
1. School of Medicine, Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, Jiangsu University
2. Shanghai East Hospital, Barry J Marshall Diagnostic and Treatment Center for Digestive Diseases, Tongji University School of Medicine
- Publication Type:Journal Article
- Keywords:
gastric cancer;
long non-coding RNA;
LINC00473;
proliferation;
migration
- From:
Chinese Journal of Clinical Laboratory Science
2019;37(5):383-388
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Abstract: Objective: cancer cells and its effects on the proliferation and migration of gastric cancer cells.
Methods:The expression level of LINC00473 in gastric cancer cells was verified by qRT-PCR system. LINC00473 siRNA segment and overexpression vector were separately transfected into gastric cancer cells by the method of lipofection. The proliferation and migration abilities of gastric cancer cells with LINC00473 knockdown or overexpression in vitro were evaluated by cell counting kit-8 (CCK8) assay, colony formation assay and Transwell migration assay. The expression levels of proteins involved in epithelial-mesenchymal transition (EMT) were examined by western blot analysis.
Results:The expression levels of LINC00473 were decreased in gastric cancer cells compared with that in human gastric epithelial cell strain GES-1 (P＜0.05). LINC00473 knockdown cells showed significant increased ability for cell growth (F=163.10, P＜0.01) and colony formation (t=3.29, P＜0.05) compared with the knockdown cells in scramble control. The results of Transwell migration assay showed that LINC00473-knockdown enhanced the migratory abilities of gastric cancer cells (t=4.68, P＜0.05). The knockdown of LINC00473 downregulated E-cadherin expression (t=4.08, P＜0.05) and upregulated N-cadherin (t=5.06, P＜0.01), Snail (t=7.69, P＜0.01) and Vimentin (t=3.82, P＜0.05) expression. Compared with the control group, LINC00473 overexpression cells showed significantly decreased cell growth (F=186.00, P＜0.01) and colony formation ability (t=3.22, P＜0.05). The results of Transwell migration assay showed that LINC00473-overexpression reduced the migratory ability of gastric cancer cells (t=5.52, P＜0.05). The overexpression of LINC00473 enhanced E-cadherin expression (t=2.90, P＜0.05) and reduced the expressions of N-cadherin (t=7.44, P＜0.01), Snail (t=2.78, P＜0.05) and Vimentin (t=4.64, P＜0.01).
Conclusion:The knockdown of LINC00473 may promote gastric cancer cell proliferation and migration in vitro by regulating EMT.
