Effects of Tim-3 on osteoclast-like cell formation and bone resorption induced by peripheral blood mononuclear cells
10.13602/j.cnki.jcls.2019.05.09
- VernacularTitle:Tim-3对单核细胞诱导破骨样细胞生成和骨吸收功能的影响
- Author:
Xiaoli DAI
1
;
Yinyin FAN
1
;
Hong ZHANG
1
;
Ting MAO
1
;
Honglei SONG
1
;
Hong DU
1
Author Information
1. Department of Clinical Laboratory, the Second Affiliated Hospital of Soochow University
- Publication Type:Journal Article
- Keywords:
T cell immunoglobulin and mucin domain 3;
rheumatoid arthritis;
osteoclast-like cell;
TRAP staining;
bone resorption
- From:
Chinese Journal of Clinical Laboratory Science
2019;37(5):353-357
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of Tim-3 on osteoclast-like cell (OLC) formation and bone resorption induced by peripheral blood mononuclear cells (PBMCs).
Methods:The expression levels of Tim-3 in of rheumatoid arthritis (RA) patients and healthy controls were detected by flow cytometry. The OLCs were induced by human PBMCs in vitro. The expression levels of tartrate-resistant acid phosphatase (TRAP), cathepsin K (CTSK) and matrix metalloproteinase 9 (MMP9) mRNAs in the formation of OLCs were detected by real-time quantitative PCR. The morphology of OLCs was observed by Wright′s staining and G-actin staining, and the number of OLCs was counted by TRAP staining. The number and area of bone resorption pits in OLCs were detected by the Corning Osteo Assay Surface.
Results:The expression levels of Tim-3 in PBMCs of RA patients ([77.31±10.66]%) were significantly higher than that of healthy controls ([51.72±16.69]%, t=7.593, P＜0.01). When PBMCs with different Tim-3 levels were induced into OLCs, the area of bone resorption pits in the high Tim-3 level group ([1.054±0.085] S/mm 2 ) were significantly lower than those in the intermediate Tim-3 level group ([1.889±0.053] S/mm 2 ) and the low Tim-3 level group ([2.763±0.066] S/mm 2 , F=9.318, P＜0.05).
Conclusion:Tim-3 may negatively regulate the bone resorption of OLCs.
