Identification and characterization of 4 Prototheca wickerhamii strains
10.13602/j.cnki.jcls.2019.04.16
- VernacularTitle:4株威克汉姆无绿藻的鉴定及基因特征分析
- Author:
Lianghui LI
1
;
Minling ZHENG
1
;
Qiuping HUANG
2
;
Cha CHEN
3
;
Pinghua QU
1
,
4
Author Information
1. Second Clinical College, Guangzhou University of Chinese Medicine
2. Maccura Biotechnology Co., Ltd.
3. Department of Laboratory Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Traditional Chinese Medicine
4. Department of Laboratory Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Prototheca;
identification;
phylogenetic analysis
- From:
Chinese Journal of Clinical Laboratory Science
2019;37(4):305-309
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To identify and characterize the 4 strains of Prototheca isolated from the clinical samples of skin or ascites samples in China.
Methods:The taxonomic position of 4 yeast-like organisms was revealed by polyphasic taxonomic approach, i.e., cultural and morphologic characteristics, commercial biochemical systems of Vitek 2 (YST kit) and Vitek matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry (MS) systems in combination with phylogenetic analysis based on the gene sequences of 16S and 28S rRNA.
Results:The 4 strains of Prototheca were characterized as cream-white, smooth, moist yeast-like colonies on Sabouraud gentamicin chloramph agar after incubation for 3 days. However, round, oval-shaped or elliptical sporangiums with mulberry-like or strawberry-like endospores were observed by optical microscope, which showed distinct differences from the general yeast species. The 4 isolates were identified as Prototheca wickerhamii with Vitek YST kits by Vitek 2 systems and Vitek MALDI-TOF MS systems. The genome for the 4 isolates was characterized with the existence of the prokaryotic 16S rRNA gene and eukaryotic 28S rRNA gene. The 16S rRNA gene sequence of the 4 strains showed more than 99.7% similarity to that of P. wickerhamii. Sequence analysis of 28S rRNA gene showed that the organisms included multiple copies of different sequences, which showed sequence similarities of 91.9% to 100% even in the same strain. The phylogenetic dendrogram based on 16S rRNA and 28S rRNA gene sequences showed that the 4 strains of Prototheca formed a cluster along with P. wickerhamii.
Conclusion:The 4 yeast-like organisms could be identified as P. wickerhamii, and 16S rRNA gene should be the suitable molecular target for the identification.
