Analysis of TCR β chain CDR3 clonetype in HBV-infected patients with coexistence of HBsAg and HBsAb
10.13602/j.cnki.jcls.2019.04.09
- VernacularTitle:HBsAg、HBsAb双阳性HBV感染者TCR β链CDR3克隆型分析
- Author:
Le CHANG
1
;
Zheng ZENG
2
;
Sa WANG
2
;
Yanfeng HOU
1
;
Zhiyan LI
1
;
Zhenru Feng
1
Author Information
1. Department of Clinical Laboratory, Peking University First Hospital
2. Department of Infectious Diseases, Peking University First Hospital
- Publication Type:Journal Article
- Keywords:
T-cell receptor;
complementarity-determining region 3;
clonotype;
HBsAg;
HBsAb
- From:
Chinese Journal of Clinical Laboratory Science
2019;37(4):278-282
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare T-cell receptor (TCR) β chain complementarity-determining region 3 (CDR3) in the patients with coexistence of HBsAg and HBsAb and other HBV infected patients.
Methods:The clonotype and diversity of CDR3 in blood of group cases (positive HBsAg and HBsAb) (n=11), control 1 (negative HBsAg and positive HBsAb) (n=10) and control 2 (positive HBsAg and negative HBsAb) (n=10) were analyzed by high-throughput TCR sequencing with Illumina HiseqX10.
Results:In the case group, the overlap rate of 6.28% (0.25%, 13.10%) was detected between any two samples, which was significantly lower than the overlap rate of 10.49% (6.20%，17.30%) seen in control 1 group (P=0.008). In control 2 group, the overlap rate of 2.60% (0.13%，13.69%) was significantly lower than control 1 group (P=0.001). There was no difference between case group and control 2 group. After pairwise comparison between the three groups, the frequency of clonotype TRBV7-2/TRBD1/TRBJ2-1 in case group was higher than that of control 1 group (P=0.029), the frequency of TRBV7-3/TRBD1/TRBJ2-7 in case group was lower than that of control 1 group (P=0.031). The difference of TRBV5-8 was significant in comparing case group with control 1 group (P=0.047). There were 14 clonotypes which had differences between case group and control 2 group in frequency. TRBV28was significant in comparing case group with control 2 group (P=0.028). For diversity, there was no difference among the three groups.
Conclusion:Clonotype TRBV7-2/TRBD1/TRBJ2-1, TRBV7-3/TRBD1/TRBJ2-7 and TRBV5-8 were associated with coexistence of HBsAg and HBsAb, but the diversity was not associated with TCR β chain CDR3.
