Infiltration and significance of tissue-resident CD8 + T cells in gastric cancer
10.13602/j.cnki.jcls.2019.04.03
- VernacularTitle:胃癌组织中驻留CD8 + T细胞的浸润程度及其意义
- Author:
Yan WANG
1
,
2
;
Huimin LU
1
,
2
;
Lujun CHEN
3
;
Binfeng LU
1
,
2
,
4
;
Jingting JIANG
1
,
2
Author Information
1. Medical College of Soochow University
2. Tumor Biological Treatment Center, the Third Affiliated Hospital of Soochow University, Engineering Technology Research Center for Tumor Immunotherapy of Jiangsu Province, Institute of Cell Therapy of Soochow University
3. Tumor Biological Treatment Center, the Third Affiliated Hospital of Soochow University, Engineering Technology Research Center for Tumor Immunotherapy of Jiangsu Province, Institute of Cell Therapy of Soochow University
4. Department of Immunology, School of Medicine, University of Pittsburgh
- Publication Type:Journal Article
- Keywords:
gastric cancer;
CD103 + CD8 + T cell;
tissue microarray;
prognosis
- From:
Chinese Journal of Clinical Laboratory Science
2019;37(4):251-256
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the degree of infiltration and distribution of tissue-resident CD8 + T cells (CD103 + CD8 + T cells) in gastric cancer tissues, and analyze the relationship between the degree of infiltration and clinicopathological features and prognosis.
Methods:Tissue microarray and immunofluorescence staining were used to examine the CD8 + T cells and CD103 + CD8 + T cells infiltration in 90 cases of gastric cancer and their adjacent normal tissues. Wilcoxon rank test was used to compare the CD8 + T cells, CD103 + CD8 + T cells infiltration and CD103 + CD8 + T cells/CD8 + T cells ratio in gastric cancer and corresponding normal tissues. The chi-square test was used to analyze the relationship between CD8 + T cells, CD103 + CD8 + T cells infiltration and CD103 + CD8 + T cells/ CD8 + T cells ratio in gastric cancer tissues and clinicopathological features of the patients. Kaplan-Meier survival analysis was performed to explore the correlation between CD8 + T cells, CD103 + CD8 + T cells infiltration and CD103 + CD8 + T cells/ CD8 + T cells ratio and overall survival. Cox model was used to analyze the correlation between different clinical parameters and prognosis of the patients.
Results:There was no significant difference for the infiltration of CD103 + CD8 + T cells between the gastric cancer tissues and adjacent normal tissues (P＞0.05). The infiltration rate of CD103 + CD8 + T cells in the cases in stage Ⅲ to Ⅳ (69.09%, 38/55) was significantly lower than that in the stage Ⅰ to Ⅱ cases (91.43%, 32/35), (χ 2 =6.175, P=0.013). There was no significant correlation between CD103 + CD8 + T cells infiltration and other clinicopathological features (P＞0.05). Kaplan-Meier survival analysis showed that the patients with high CD103 + CD8 + T cells infiltration showed significantly longer overall survival than the patients with low CD103 + CD8 + T cells infiltration (HR=2.187, 95%CI: 1.062-4.500, P=0.033 6). Multivariate Cox model analysis indicated that tumor diameter (HR=2.031, 95%CI: 1.163-3.546, P=0.013) and CD103 + CD8 + T cells infiltration (HR=0.516, 95%CI: 0.285-0.934, P=0.029) were independent prognostic factors for gastric cancer.
Conclusion:CD103 + CD8 + T cells in gastric cancer tissues should be associated with good prognosis, suggesting that they play an important role in the inhibition of gastric carcinogenesis and development, and can be used as an important factor for the prognosis evaluation of the patients with gastric cancer.
