Development and evaluation of light-initiated chemiluminescent assay for quantitation of milk-specific IgG 4 antibody in human serum
10.13602/j.cnki.jcls.2019.04.01
- VernacularTitle:牛奶过敏单组分特异性IgG 4 抗体光激化学发光定量检测方法的建立和评价
- Author:
Yaqiong CUI
1
;
Junpu LI
2
;
Shaoshen LI
3
;
Liuxu LI
2
;
Lunhui HUANG
2
;
Huiqiang LI
2
;
Weizhen GAO
1
Author Information
1. School of Basic Medicine, Tianjin Medical University
2. School of Medical Laboratory, Tianjin Medical University
3. Department of Clinical Laboratory, Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital
- Publication Type:Journal Article
- Keywords:
specific IgG 4 antibody;
Bos d 5;
light-initiated chemiluminescent assay;
performance evaluation
- From:
Chinese Journal of Clinical Laboratory Science
2019;37(4):241-245
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop and evaluate a beads-based light-initiated chemiluminescent assay (LICA) for quantitation of cow milk component (Bos d 5) specific IgG 4 antibody in human serum.
Methods:The sIgG 4 -LICA was performed by incubated serum samples with biotinylated allergens, emission beads coated with mouse anti-human IgG 4 antibody and streptavidin-coated sensitizer beads. The reaction conditions of sIgG 4 -LICA were optimized and the analytical performance was evaluated.
Results:The precision of intra-assay, within-day and inter-assay (coefficient of variation) were 1.78% to 3.13%, 6.65% to 8.41% and 7.94% to 12.30%, respectively. The functional sensitivity of this assay was 4.71 ng/mL. For the linear range, the sIgG 4 -LICA had a good linear relationship within the range between 28.13 and 1 800 ng/mL, and the linear regression equation was Y=0.98X-1.31(r 2 =0.997). Maximum dilution limit was 1∶64. The disturbing rates measured by adding hemoglobin, triacylglycerol, total bilirubin, acid resistance and biotin to human sera with different concentrations of Bos d 5 sIgG 4 were from -6.38% to 8.60%.
Conclusions:The sIgG 4 -LICA introduced in this study was demonstrated to have effective performance for quantitation of allergen-specific IgG 4 and can meet the need of clinical requirement.
