Application of thinprep liquid-based cytologic test combined with immunofluorescence staining in the detection of urinary podocytes
10.13602/j.cnki.jcls.2019.03.03
- VernacularTitle:液基薄层细胞学技术联合免疫荧光染色在尿足细胞检测中的应用
- Author:
Xinping SUN
1
;
Juhua DAI
1
;
Jie ZHANG
2
Author Information
1. Department of Clinical Laboratory, Peking University International Hospital
2. Department of Clinical Laboratory, Peking University Third Hospital
- Publication Type:Journal Article
- Keywords:
liquid-based thinprep cytologic test;
conventional centrifugal smear;
indirect immunofluorescence staining;
podocyte
- From:
Chinese Journal of Clinical Laboratory Science
2019;37(3):173-176
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare the indirect immunofluorescence staining effect of urinary podocytes by thinprep liquid-based cytologic test (TCT) with that by the conventional centrifugal smear method, and explore its clinical application value.
Methods:The morning urine samples from 50 patients with type 2 diabetic nephropathy and 14 healthy controls were smeared with the TCT and conventional centrifugation method, respectively, and then the indirect immunofluorescence staining were performed to observe the morphology of podocytes.
Results:For 64 urine samples, the satisfactory rate of TCT smears (85.94%) was significantly higher than that of conventional smears (50.00%), and the podocyte detection rate of TCT smears (73.47%) was also significantly higher than that of conventional smears (51.02%) (P＜0.05). When urinary podocytes of the same patient were positive by both methods, the reading effect of TCT smears was obviously superior to that of conventional smears.
Conclusion:The TCT combined with indirect immunofluorescence staining is obviously superior to the conventional centrifugal smear method in the podocyte diagnosis of urine samples.
