Evaluation of cultivated and wild Herba Epimedii using UHPLC-PDA-Q-TOF/MSE
10.16438/j.0513-4870.2019-0885
- VernacularTitle:基于UHPLC-PDA-Q-TOF/MSE技术的栽培淫羊藿质量研究
- Author:
Ming ZHOU
1
,
2
,
3
;
Wei ZHENG
4
;
Bao-lin GUO
5
;
An-jia CHEN
1
;
Bai-ping MA
4
Author Information
1. Shanxi Medical University, Taiyuan 030001, China
2. Institute of Radiation Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China
3. Institute of Medicinal Plant Development, Chinese Academy of Medical Science, Peking Union Medical College, Beijing 100193, China
4. Institute of Radiation Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China
5. Institute of Medicinal Plant Development, Chinese Academy of Medical Science, Peking Union Medical College, Beijing 100193, China
- Publication Type:Research Article
- Keywords:
italic>Epimedium;
cultivation;
UHPLC-PDA-Q-TOF/MSE;
quality study
- From:
Acta Pharmaceutica Sinica
2020;55(5):995-1003
- CountryChina
- Language:Chinese
-
Abstract:
Qualitative and relatively quantitative methods were used to study the quality of cultivated Epimedium sagittatum (Sieb. et Zucc.) Maxim., Epimedium myrianthum Stearn, and Epimedium pubescens Maxim by ultra high-performance liquid chromatography coupled with photo-diode array and quadrupole time-of-flight mass spectrometry (UHPLC-PDA-Q-TOF/MSE). Thirty-two compounds in cultivated and wild samples of E. sagittatum, E. myrianthum and E. pubescens were identified using UHPLC-Q-TOF/MSE combined with the UNIFI data analysis platform. Principal component analysis (PCA) was used to compare the cultivated and wild samples of these three species. The results show that the chemical compositions of cultivated samples were consistent with the corresponding wild samples. UHPLC-PDA was used to determine the relative content of 12 flavonoids as well as total flavonoids in all samples. The results show that the relative chemical content of these flavonoids in cultivated and wild samples is similar and the quality of cultivated Epimedium is more stable. These qualitative and relatively quantitative methods using UHPLC-PDA-Q-TOF/MSE combined with the UNIFI data analysis platform and PCA can be used to study the quality of cultivated Herba Epimedii. This research provides a scientific basis for the cultivation and rational development and utilization of Epimedium medicinal materials.
